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高多重的CRISPR基编辑在Streptomycetes中的系统分析

Christopher M Whitford¹, Tetiana Gren¹, Emilia Palazzotto¹

¹The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.

ACS synthetic biology

|July 4, 2023

概括

在Streptomyces coelicolor中,CRISPR-mcBEST能够通过同时准多个基因集群来实现多重基因编辑. 这种先进的CRISPR基编辑技术为Streptomyces的高通量工程提供了潜力.

科学领域:

微生物学微生物学
分子生物学分子生物学
合成生物学合成生物学

背景情况:

像CRISPR-BEST这样的CRISPR基编辑工具,已经简化了链球菌的基因操纵.
多重基因组编辑是有利的,特别是在基因组不稳定的物种中.

研究的目的:

在Streptomyces coelicolor中使用基于Csy4的CRISPR-mcBEST进行扩大规模的多重基因组编辑.
评估多重编辑在各种尺度上的性能,针对所有28个专门的代谢物生物合成基因集群.

主要方法:

使用基于Csy4的CRISPR-mcBEST进行Streptomyces coelicolor的多重基因组编辑.
对9,18,28个基因集群进行了同时准.
进行了多组学分析,以评估系统范围的影响.

主要成果:

成功演示了扩大多重基因组编辑,在单个实验中针对所有28个基因集群.
提供了基于Csy4的多重基因组编辑的性能和可扩展性的见解.
多组学分析揭示了CRISPR-mcBEST的潜力和局限性.

结论:

CRISPR-mcBEST是一个强大的工具,用于Streptomyces多重基因组编辑.
这项技术对Streptomyces和其他微生物底盘的高通量工程具有重大潜力.
需要进一步开发,以克服已识别的瓶,以便更广泛地应用.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?这就是CRISPR-BEST.这种病名为Streptomyces.基础编辑器基础编辑器赛蒂丁脱胺酶的作用多重基因组工程是多重基因组工程.

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