人体羊纤维细胞的IL-33/ST2轴参与分娩时的炎症反应

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Isolation of Leukocytes from the Human Maternal-fetal Interface

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Published on: May 21, 2015

Wen-Jia Lei^1,2, Fan Zhang^1,2, Yi-Kai Lin^1,2

¹Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P. R. China.

Molecular medicine (Cambridge, Mass.)

|July 4, 2023

概括

介质素-33 (IL-33) 和ST2轴在分娩期间在人类胎儿膜中活跃. 这种途径促进炎症,并有助于早产,建议它作为治疗点.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

胎儿膜的炎症对于分娩开始至关重要.
介素-33 (IL-33) 是一种通过ST2受体起作用的炎症性细胞因子.
人类分娩中的IL-33/ST2轴的作用以前是未知的.

研究的目的:

在分娩期间调查IL-33/ST2轴在人类胎儿膜中的存在和作用.
为了确定IL-33/ST2激活是否有助于分娩中的炎症反应.
探索针对这一轴进行早产治疗的潜力.

主要方法:

转录基因测序,qPCR,西式涂抹和免疫组织化学用于分析人类羊膜样本.
培养了初级人类羊纤维细胞,以研究IL-33/ST2轴调节和功能.
用小鼠模型来评估IL-33在分娩中的体内作用.

主要成果:

IL-33和ST2存在于人类羊膜纤维细胞和上皮细胞中,纤维细胞的丰度更高.
在终期和早产期间,IL-33和ST2的表达在羊膜中显著增加.
炎症媒介诱导纤维细胞中的IL-33表达,而纤维细胞则通过ST2受体产生炎症因子 (IL-1β,IL-6,PGE2).
在小鼠中,使用IL-33诱导过早分娩.

结论:

在分娩期间,IL-33/ST2轴在人类羊纤维细胞中存在并被激活.
这个轴的激活促进炎症,并有助于早产.
准IL-33/ST2轴可能是预防早产的治疗策略.

关键词:

羊膜膜是什么意思羊膜是什么意思在COX-2中,COX-2是最常见的.这里有IL-33和IL-33.分割的方法分割的方法 ST2 ST2 ST2 ST2 ST2 ST2 ST2 ST2 ST2 ST2 ST2 ST2 ST3 ST4 ST4 ST5 ST6 ST7 ST7 ST7

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