Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

67
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
67

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chiral plasmonic Cu<sub>2-x</sub>S quantum dots enable chirality-dependent fluorescent recognition of tryptophan enantiomers.

Talanta·2026
Same author

Harnessing the Versatility of SAM-Dependent Enzymes: From Mechanism to Application.

Chembiochem : a European journal of chemical biology·2026
Same author

Defined Nylon Oligomers Enable Mechanistic Insight Into Enzymatic Polyamide Depolymerization.

ChemSusChem·2026
Same author

Electrochemistry-Driven Strain Release of Bicyclo[1.1.0]Butanes for the Synthesis of Cyclobutenes.

The Journal of organic chemistry·2026
Same author

Genuine Directed Evolution In Test Tube (GENie).

bioRxiv : the preprint server for biology·2026
Same author

Air Pollution Elevates the Risks of Incident Hypertension, Subsequent Comorbidities Progression, and Prognosis: A Multistate Analysis.

American journal of preventive medicine·2026

相关实验视频

Updated: Jul 24, 2025

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
06:16

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

20.6K

工程全方位细胞酶用于生物乙醇生产.

Minghui Wang1, Haiyang Cui2, Chenlei Gu1

  • 1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, No. 2 Xuelin Road, Nanjing 210097, China.

ACS synthetic biology
|July 5, 2023
PubMed
概括

工程细胞酶变体通过克服乙醇抑制来显著提高生物乙醇生产. 这种蛋白质工程方法提高了酶稳定性和催化效率,提高了生物燃料产量.

关键词:
生物乙醇生物乙醇的使用细胞质的细胞质.发酵 发酵 发酵 发酵优化了2GenReP的使用情况.一个强大的酶.

更多相关视频

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

11.3K

相关实验视频

Last Updated: Jul 24, 2025

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
06:16

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Published on: August 13, 2011

20.6K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

11.3K

科学领域:

  • 生物技术是生物技术.
  • 生物能源工程 生物能源工程
  • 酵素工程是什么意思 酵素工程

背景情况:

  • 从生物质中生产生物乙醇对于减少原油消耗和环境影响至关重要.
  • 细胞分解酶的稳定性和水解效率是生物乙醇发酵的关键局限性,通常因乙醇度的增加而受到阻碍.
  • 乙醇对酶的非活性化会降低最终的生物乙醇产量,因此需要制定提高酶性能的策略.

研究的目的:

  • 进化细胞酶CBHI (碳水化合物结合域I) 以提高生物乙醇生产的性能,使用优化的两基因重组过程 (2GenReP).
  • 开发具有对乙醇,有机溶剂抑制剂的增强耐药性和在同时糖化和发酵 (SSF) 过程中提高稳定性的细胞酶变体.

主要方法:

  • 利用一个优化的两基因重组过程 (2GenReP) 来设计细胞酶CBHI.
  • 选并确定了两种高度有效的CBHI变体,被指定为R2和R4.
  • 在各种条件下评估了进化变体的催化效率 (kcat/KM) 和稳定性,包括乙醇和有机溶剂的存在.

主要成果:

  • 两种全方位的CBHI变体R2和R4已成功演化,表现出更好的乙醇耐药性,有机溶剂耐受性和SSF稳定性.
  • CBHI变种R4在乙醇存在或不存在的情况下,催化效率 (kcat/KM) 增加了7.0至34.5倍.
  • 将进化CBHI R2和R4纳入1G生物乙醇工艺,导致乙醇产量显著改善,比对照组高出10.27% (6.7 g/L).

结论:

  • 2GenReP方法有效地产生了强大的细胞酶变体,用于实际的生物乙醇发酵.
  • 进化的CBHI变种 (R2和R4) 提供了卓越的稳定性和催化效率,克服了当前生物乙醇生产工艺的关键局限性.
  • 这种蛋白质工程策略是可转移的,并有可能开发用于生物转化和生物能源应用的多功能酶.