相关实验视频

Updated: Jul 24, 2025

Viral Tracing of Genetically Defined Neural Circuitry

Viral Tracing of Genetically Defined Neural Circuitry

Published on: October 17, 2012

[IL-33基因重组和表达在体外没有影响狂犬病病毒的表型特征]

Ting Gao¹, Zhizhong Mi², Ming Sun²

¹Basic Medical College of Dali University, Dali Nursing Vocational College, Dali 671000, China.

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

|July 5, 2023

概括

这项研究创造了一种重组狂犬病病毒 (rLBNSE-IL33),该病毒过度表达Interleukin-33 (IL-33). 过度表达IL-33并没有显著改变复合狂犬病病毒.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

狂犬病病毒是一个重大的公共卫生问题.
介素-33 (IL-33) 是一种具有免疫调节功能的细胞因子.
了解病毒修饰对于开发新疗法至关重要.

研究的目的:

为了设计一种重组狂犬病病毒 (rLBNSE-IL33),该病毒会过度表达IL-33.
评估IL-33过度表达对狂犬病病毒的体外表型影响.

主要方法:

用逆遗传学将基因放大并插入LBNSE基因组.
用复合病毒和父病毒感染BSR和小鼠NA细胞.
通过测序和中和试验进行稳定性评估.
病毒定位,生长曲线分析,细胞毒性测定和ELISA用于IL-33检测.

主要成果:

获救的rLBNSE-IL33在10代以上表现出遗传稳定性,标位约为10^8FFU/mL.
在rLBNSE-IL33感染细胞中证实了高水平,剂量依赖的IL-33表达.
在rLBNSE-IL33和父母LBNSE菌株之间没有观察到病毒生长动力或细胞活性的显著差异.

结论:

在复合狂犬病病毒中IL-33的过度表达不会显著影响其体外表型特征.
改造的rLBNSE-IL33病毒保持稳定性和生长特性,与野生类型菌株相美.

更多相关视频

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.