利醇调节了人脑内皮细胞的激活,透性和完整性,这些细胞是由Plasmodium falciparum诱导的
Adriana A Marin1,2, Annette Juillard2, Alejandro M Katzin1
1Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Parasitologia, São Paulo, SP, Brasil.
Memorias do Instituto Oswaldo Cruz
|July 5, 2023
概括
醇 (POH) 有效地保护人类大脑内皮细胞免受Plasmodium falciparum感染. 它可以防止透性增加,并保持脑血屏障的完整性,这对于脑疟疾治疗至关重要.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 大脑疟疾 (CM) 是一种严重的脑血管病变,由菌引起.
- 寄生性红细胞 (pRBC) 在大脑微血管中被封存,导致炎症和血脑屏障 (BBB) 崩.
- 烯,像危险醇 (POH),在实验模型中显示有望预防CM相关的病理.
研究的目的:
- 为了研究危险醇 (POH) 对人类大脑内皮细胞 (HBEC) 的影响,与pRBCs共同培养.
- 评估POH对内皮细胞激活,完整性和透性的影响.
主要方法:
- 定量免疫光评估了紧结蛋白 (TJPs) 和粘附分子 (ICAM-1,VCAM-1).
- 流细胞计测量了从HBECs中释放的微囊 (MV).
- 超内皮电阻 (TEER) 评估了POH恢复单层透性的能力.
主要成果:
- POH显著抑制了PRBC诱导的ICAM-1和VCAM-1的上调.
- 在HBEC单层中,POH减少了MV释放,并改善了TEER.
- POH恢复了关键TJPs的分布,包括VE-cadherin,Occludin和JAM-A.
结论:
- POH是一种强大的单烯,可以有效地抵消Plasmodium falciparum诱导的大脑内皮细胞的变化.
- POH可以防止内皮激活,增加透性,以及与CM病原发生相关的完整性改变.
- 通过保护大脑内皮,POH显示了治疗大脑疟疾的治疗潜力.
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