家庭用聚烯是否适用于塑化薄片大脑?
L S Juvenato1,2, Y F Monteiro1, R P Miranda1
1Laboratório de Plastinação, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
国内聚P18是大脑切片塑化的一种可行的替代品,提供了一个具有成本效益的解决方案. 这项研究支持巴西塑料化技术的扩展,利用当地采购的材料.
科学领域:
- 人体解剖学 解剖学 解剖学
- 生物材料科学 生物材料科学
- 医疗教育 医学教育
背景情况:
- 塑化使用聚合物浸来保存生物组织,聚对大脑样本来说是理想的.
- 目前的塑料化材料往往是进口和昂贵的,阻碍了更广泛的采用,特别是在巴西.
- 开发国内的聚合物替代品可以显著扩大塑化在教学和研究中的应用.
研究的目的:
- 评估在牛脑切片塑化中使用国产聚烯作为进口Biodur® (P40) 的替代品的可行性.
- 评估家用聚P18和Cristalan© (C1-3) 聚合物的适用于大脑塑化.
- 为了比较国内聚合物的有效性与标准P40聚.
主要方法:
- 牛脑切片 (2毫米厚) 被准备好并接受标准的塑化方案:固定,脱水,强制浸和固化.
- 用每个聚类型塑化了15个切片:P40 (对照),P18和C1-3.
- 使用标准化的照片来比较浸前和固化后的切片,以评估收缩等变化.
主要成果:
- 与标准P40.0相比,国内聚P18的百分比收缩没有显著差异.
- 克里斯塔兰© (C聚合物) 的固化时间太短,即使没有启动剂,也无法有效浸.
- 国内生产的P18聚塑料被证明是塑化工艺的可行选择.
结论:
- 国内聚P18是一种适合且可行的替代品,可以替代进口的Biodur® (P40) 用于牛脑切片塑化.
- 像P18这样的国内聚合物的成本效益可以促进巴西塑化工艺的扩张.
- 可能需要进一步的研究来优化其他国内聚合物的使用或解决Cristalan©观察到的局限性.
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