在线状真菌和宏真菌中应用CRISPR:从组件功能到发展潜力
Jia-Yu Shen1, Qunfei Zhao1, Qing-Li He1
1Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
ACS synthetic biology
|July 5, 2023
概括
状真菌对于生产有用的化合物是有价值的. CRISPR/Cas9基因编辑系统提供了一个有希望的解决方案,以克服修改这些真菌用于合成生物学应用的挑战.
科学领域:
- 菌类学和合成生物学 合成生物学
- 分子生物学和基因工程分子生物学和基因工程
背景情况:
- 纤维状真菌和宏真菌具有产生有价值的二次代谢产物,酶和天然产品的巨大潜力.
- 有效的基因改造技术对于利用合成生物学中的真菌能力至关重要.
- 诸如真菌异菌症和主导非同类末端结合 (NHEJ) 修复等挑战阻碍了高效的基因编辑.
研究的目的:
- 审查CRISPR/Cas9系统在线状真菌和巨型真菌中进行基因改造的应用和发展.
- 讨论CRISPR/Cas9在真菌基因编辑中的组件,挑战和未来潜力.
主要方法:
- 关于CRISPR/Cas9系统组件 (cas9,sgRNA,促进物,查标记物) 的现有文献的审查.
- 对CRISPR/Cas9系统在线状真菌和宏观真菌中的有效性和局限性的分析.
- 探索CRISPR/Cas9的发展和适应用于真菌遗传工程.
主要成果:
- 克里斯普尔/卡斯9系统已经成为一种强大的菌基因改造工具.
- 了解和优化CRISPR/Cas9组件是提高基因编辑效率的关键.
- 该系统显示出巨大的潜力,尽管在特定的真菌物种存在挑战.
结论:
- 克里斯普尔/卡斯9技术对于在线状真菌和宏观真菌中推进基因工程至关重要.
- 需要进一步研究克服修复机制主导性和异构造的方法.
- 克里斯普尔/Cas9系统对未来使用真菌的合成生物学应用具有重大前景.
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