通过MshH精确控制Csr sRNA水平,增强了成年小鼠中的Vibrio cholerae殖民
Mengting Shi1,2,3, Jinjie Ye1, Fenxia Fan4
1Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Hangzhou, Zhejiang, China.
Applied and environmental microbiology
|July 5, 2023
概括
在小鼠中的Vibrio cholerae殖民需要精确控制CsrB,CsrC和CsrDRNA水平. MshH蛋白调节这些水平,影响细菌的生存和传播.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 霍乱病毒 (Vibrio cholerae) 引起霍乱,肠道殖民对于其致病性和传播至关重要.
- 包括CsrA,CsrB,CsrC和CsrD在内的Csr (碳储存调节器) 系统是许多细菌中关键的转录后调节器.
- 了解V. cholerae殖民机制对于开发有效的干预措施至关重要.
研究的目的:
- 为了研究MshH蛋白在V. cholerae肠道殖民中的作用.
- 为了阐明涉及MshH,CsrB,CsrC,CsrD和CsrA在V.cholerae殖民中的监管途径.
- 为了确定V. cholerae如何适应成年老鼠肠道.
主要方法:
- 对V. cholerae的基因操纵,包括mshH,csrB和csrD的删除突变.
- 使用RT-qPCR对CsrB,CsrC和CsrDRNA水平的量化.
- 在成年小鼠的肠道中评估V.cholerae的殖民化.
- 对CsrRNAs的MshH-依赖性降解和CsrA-依赖性稳定性的分析.
主要成果:
- 删除mshH导致在成年小鼠的V. cholerae肠道殖民中的显著缺陷.
- mshH删除导致CsrB和CsrDRNA水平增加,CsrCRNA水平降低.
- 删除CsrB和CsrD恢复了 mshH突变体中的殖民缺陷和CsrC水平.
- MshH主要控制CsrB和CsrDRNA的降解,而CsrA则稳定CsrCRNA.
结论:
- 通过MshH-CsrB/C/D-CsrA通路精确调节CsrB,CsrC和CsrDRNA水平,对于成年小鼠的V. cholerae殖民是必不可少的.
- 霍乱病毒采用不同的策略来控制CsrRNA的丰富性,在宿主肠道中赋予生存优势.
- 这项研究揭示了一种对V. cholerae适应性和传染性至关重要的新型调节机制.
相关概念视频
01:28Regulation of Bacterial Virulence
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
01:25Cholera
Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...