在柬埔寨,日本脑炎病毒载体物种的种类和空间分布
Rutaiwan Dusadeepong1, Pierre-Olivier Maquart1, Mallorie Hide2,3,4
1Medical and Veterinary Entomology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Medical and veterinary entomology
|July 5, 2023
概括
在柬埔寨的日本脑炎病毒 (JEV) 载体蚊子,Culex vishnui,Cx. pseudovishnui,以及Cx. 这两种类型. 基因分析的使用被确定为tritaeniorhynchus. 这些JEV载体在柬埔寨各地广泛分布,在不同的环境中.
科学领域:
- 医学昆虫学 医学昆虫学
- 分子遗传学分子遗传学
- 公共卫生 公共卫生
背景情况:
- 尽管有疫苗接种努力,日本脑炎 (JE) 仍然是东南亚的重大公共卫生问题.
- 昆虫属Culex的蚊子是日本脑炎病毒 (JEV) 的主要载体.
- 准确识别柬埔寨关键的JEV载体物种,特别是Vishnui亚组 (Culex vishnui,Cx. 假名维什努伊,Cx. 由于形态上的相似性,这是一个具有挑战性的挑战.
研究的目的:
- 识别和绘制柬埔寨三个主要JEV载体物种的分布图:Culex vishnui,Cx. pseudovishnui,以及Cx. 这两种类型. 三年级鼻. 三年级鼻.
- 为了澄清这些关键蚊子载体的遗传关系和地理分布.
主要方法:
- 在柬埔寨各种环境中广泛采集蚊子样本.
- 使用最大概率方法对细胞染色体c氧化酶子单元I (coI) 基因进行基因基因分析.
- 植物地理分析以确定物种分布模式.
主要成果:
- 遗传学分析证实了Cx.的不同类型. 三级鼻和一个Cx.的分类. 在Vishnui和Cx. pseudovishnui,与Cx. 一起使用. 在Cx. pseudovishnui中嵌套的假象. 维什努伊. 这是一个.
- 遗传地理分析显示,Vishnui亚群在柬埔寨的分布是相似的和广泛的.
- 在Cx.x. pseudovishnui主要在森林地区发现,而Cx. 三级喉 (tritaeniorhynchus) 和Cx. 这两种. 维什努伊在农村,郊区和城市环境中存在.
结论:
- 该研究使用分子方法成功区分了三个主要的JEV载体物种.
- 具有JEV能力的载体的广泛分布,包括Cx. 假名维什努伊,Cx. 维什努伊,以及Cx. 在柬埔寨, tritaeniorhynchus,对JE传播构成持续的风险.
- 了解矢量分布对于有针对性的矢量控制策略和JE预防工作至关重要.
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