狗中ESBL产生大肠杆菌的肠道殖民与独特的微生物组和抗体组合有关
Paul B Stege1, Joost Hordijk2,3, Arnar K S Sandholt4
1Department of Medical Microbiology, UMC Utrecht, Utrecht University, Utrecht, The Netherlands.
Microbiology spectrum
|July 5, 2023
概括
狗肠道微生物组随着扩展光谱β-乳糖酶产生的大肠杆菌 (ESBL-EC) 携带而发生变化. 当狗携带ESBL-EC时,特定的细菌和抗菌素耐药性基因增加,突出显示伴侣动物的微生物重要性.
科学领域:
- 兽医微生物学 兽医微生物学
- 微生物生态学 微生物生态学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 肠道微生物组作为一种多药耐药性病原体的储存库,如扩展谱β-乳糖酶产生埃舍里希亚大肠杆菌 (ESBL-EC).
- 狗在ESBL-EC运输中具有很高的流行率,随着时间的推移,其运输身份波动.
- 了解狗肠道微生物组合和ESBL-EC殖民之间的联系对于公共卫生至关重要.
研究的目的:
- 调查狗的ESBL-EC载体与其肠道微生物组和抗体组的变化之间的关联.
- 确定特定的细菌属和与伴侣犬ESBL-EC殖民相关的抗菌素耐药性基因.
主要方法:
- 在6周内从57只伴侣狗中长度取样 (每只狗4个样本).
- 使用选择性培养和PCR检测ESBL-EC运输.
- 通过16S rRNA基因分析分析肠道微生物组合的分析.
- 使用抗体捕获测序方法 (ResCap) 对抗体的表征.
主要成果:
- 在狗群中观察到ESBL-EC携带的高患病率.
- 在ESBL-EC的运输与增加的Clostridium sensu stricto 1*,Enterococcus*,Lactococcus*和Escherichia-Shigella*的丰度有显著的相关性.
- 在ESBL-EC携带和抗菌素耐药性基因丰富度增加之间发现了关联,包括*cmlA*, *dfrA*, *dhfR*, *floR*和 *sul3*.
结论:
- 狗的ESBL-EC运输与独特的肠道微生物组和抗体组合有关.
- 伴侣动物中微生物多样性的变化可能表明抗微生物耐药细菌的殖民和特定抗微生物耐药基因的选择.
- 这项研究强调了监测伴侣动物中的微生物群落的重要性,以了解病原体储存和耐药性基因传播.
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