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129 BIO 3B被归类为129 BIO 3B的乳球菌
1Department of Microbiology, Tokyo Medical University, 6-1-1 Shinjuku-ku, Shinjuku, Tokyo 160-8402, Japan.
Bioscience of microbiota, food and health
|July 5, 2023
概括
遗传学分析将益生菌Enterococcus faecium 129 BIO 3B及其对安培素耐药的变体重新归类为乳糖菌. 这些菌株缺乏关键的毒性基因,已被证实可以安全使用益生菌.
科学领域:
- 微生物学 微生物学
- 益生菌 益生菌 益生菌
- 细菌的分类学 细菌的分类学
背景情况:
- 菌体129 BIO 3B有很长的安全益生菌使用的历史.
- 对抗万科胺素耐药的Enterococcus物种的新兴担忧需要对E. faecium菌株进行重新评估.
- 一些具有较低病原性潜力的E. faecium菌株已被重新分类为Enterococcus lactis.
研究的目的:
- 调查E. faecium 129 BIO 3B和E. faecium 129 BIO 3B-R.的遗传学分类和安全性
- 为了确定这些菌株是否与新定义的E. lactis物种一致.
- 评估这些菌株的益生菌安全概况.
主要方法:
- 质谱和基本局部对齐搜索工具 (BLAST) 分析.
- 多部位序列类型化 (MLST).
- 整体基因组相关性指数 (OGRI) 的计算.
- 对特异物种基因放大和最小抑制度 (MIC) 测试安培素.
主要成果:
- 质谱和BLAST无法区分3B和3B-R菌株.
- MLST确定了3B和3B-R菌株的序列类型与E. lactis.相一致.
- 在OGRI分析中,3B和3B-R与E. lactis.有很高的同源性.
- 基因放大证实了E. lactis特定标记物的存在.
- 对于3B的安皮西林MIC为2μg/mL,在益生菌的安全范围内.
- 除了fms21之外,没有检测到任何致病基因.
结论:
- E. faecium 129 BIO 3B和E. faecium 129 BIO 3B-R在遗传学上被归类为乳腺菌.
- 缺乏显著的致病基因支持它们对益生菌应用的安全性.
- 这次重新分类澄清了长期使用的益生菌菌株的分类学地位.
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