从CH4中使用甲类植物的P3HB:生物反应器的方面,发酵过程和为成本有效的生物聚合物生产建模的模型
Parya Safaeian1, Fatemeh Yazdian1, Kianoush Khosravi-Darani2
1Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Frontiers in bioengineering and biotechnology
|July 5, 2023
概括
聚β-基酸盐 (P3HB) 生物塑料为化石燃料提供了可生物降解的替代品. 研究表明,甲型细菌可以使用天然气高效地产生P3HB,而VTLB生物反应器显示出扩大规模的希望.
科学领域:
- 生物技术和生物材料科学.
- 生物聚合物的微生物生产.
背景情况:
- 聚β-基酸盐 (P3HB) 是一种可生物降解的生物塑料,由微生物作为能量储存化合物生产.
- 用P3HB取代不可降解的化石塑料可以减轻环境污染,特别是在海洋生态系统中.
- 具有成本效益的P3HB生产对于其广泛采用至关重要,需要使用廉价的碳来源.
研究的目的:
- 通过使用甲型细菌和甲 (CH4) 作为碳源来研究P3HB的生产.
- 评估和比较不同的生物反应器配置,以实现高效的P3HB合成.
- 优化使用天然气作为可持续原料的P3HB生产.
主要方法:
- 培养一种用于细胞内P3HB积累的甲otrophic联盟.
- 五种生物反应器类型的比较:连续水箱反应器 (CSTR),强制液体垂直循环生物反应器 (VTLB),强制液体水平管状循环生物反应器 (HTLB),空运 (AL) 发酵器和气泡柱 (BC) 发酵器.
- 分析甲转化,kLa值,生产率以及每个生物反应堆的运行优缺点.
主要成果:
- 该研究的重点是利用天然气或沼气中的甲 (CH4) 作为P3HB生产的成本有效的碳来源.
- 强制液体垂直环生物反应器 (VTLB) 在优化条件下表现出卓越的性能.
- 在VTLB设置中使用Methylocystis hirsuta,细胞达到细胞干质量的51.6%的P3HB积累.
结论:
- 甲是生产P3HB生物塑料的可行和可持续原料.
- VTLB生物反应器是一个有前途的系统,用于高效和可扩展的P3HB从甲生产.
- 优化的微生物发酵使用甲类植物提供了一个成本效益的途径,可生物降解塑料,减少对化石燃料和农产品的依赖.
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