相关实验视频

Updated: Jul 24, 2025

A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

Published on: April 30, 2015

快速准备肺样本用于电子显微镜观测肺内皮质甘油的肺样本

Mone Wakatsuki¹, Takashi Takaki^2,3, Akira Ushiyama⁴

¹Department of Perioperative Medicine, Division of Anesthesiology, Showa University School of Dentistry, Tokyo, Japan.

Medical molecular morphology

|July 5, 2023

概括

一种新方法简化了可视化血管血糖 (GCX),这对于诊断血管疾病至关重要. 这种技术保留了脆弱的GCX结构,减少了准备时间,并有助于血管病变研究.

科学领域:

血管生物学血管生物学
生物医学成像技术生物医学成像技术
历史学历史学历史学

背景情况:

葡萄糖 (GCX) 是一个重要的血管层,调节透性.
GCX降解与各种血管病变有关,使其可视化在诊断上具有重要意义.
保护脆弱的GCX结构需要专门的固定技术.

研究的目的:

开发和验证一种可行的方法来可视化肺组织中的葡萄糖.
为了比较一种新的固定方法与用于GCX成像的传统技术.
在败血症模型中评估GCX完整性.

主要方法:

来自小鼠的肺组织样本被脱气并使用Alcian蓝 (ALB) 溶液固定.
沉浸固定标本使用传输和扫描电子显微镜可视化.
污泥老鼠标本作为GCX完整性的控制.

主要成果:

新的ALB浸泡固定成功可视化了GCX层,与透固定可比.
败血症小鼠标本显示球形GCX聚合物和减少GCX密度.
新方法显著减少了样本准备时间,从6天减少到2天.

结论:

开发的Alcian蓝色浸泡方法是有效的可视化肺组织中的葡萄糖.
这种技术为人类样本分析提供了更快,潜在的应用方法.
这些发现有助于理解血管病变和败血症中的GCX变化.

关键词:

快速准备的准备工作.葡萄糖葡萄糖是什么葡萄糖是什么沉浸固定装置的沉浸固定方式肺组织的肺组织.这就是SEM SEM.败血症这是一种败血症.这就是TEMEM.

更多相关视频

Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition

Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition

Published on: March 3, 2023

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

相关概念视频

Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...