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相关概念视频

The Electron Transport Chain01:30

The Electron Transport Chain

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The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
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Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

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The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
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Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

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During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
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Mitochondrial Membranes01:45

Mitochondrial Membranes

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A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
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The Supercomplexes in the Crista Membrane01:41

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The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
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ATP Synthase: Mechanism01:48

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In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
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Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
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三四氨酸-协调双二鲁 (II) 复合物诱导线粒体功能障碍

Richard J Mitchell1, Anitha S Gowda1, Alexander G Olivelli1

  • 1Department of Chemistry, University of Kentucky, 505 Rose Street, Lexington, Kentucky 40506, United States.

Inorganic chemistry
|July 5, 2023
PubMed
概括
此摘要是机器生成的。

新的 (Ru(II)) 化合物向癌细胞线粒体. 化合物3选择性地破坏线粒体膜,在斑马鱼胚胎中提供了有希望的抗癌策略,降低了毒性.

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科学领域:

  • 生物化学 生物化学
  • 药用化学 医学化学
  • 癌症生物学 癌症生物学

背景情况:

  • 癌细胞主要使用糖解,但依赖于线粒体进行转移生物能量.
  • 线粒体在细胞死亡调节中的作用使它们成为关键的抗癌点.
  • 线粒体功能障碍是癌症治疗的新兴策略.

研究的目的:

  • 为了合成和生物学描述新型的含有三基的双基 (Ru(II)) 化合物.
  • 研究连接体替代剂对Ru (II) 化合物的抗癌活性的影响.
  • 评估这些Ru(II) 化合物作为向抗癌剂的潜力.

主要方法:

  • 合成Ru(II) 化合物,其中含有不同类型的双二和二联体.
  • 使用流细胞计来评估线粒体膜潜力的生物学表征.
  • 针对癌细胞的体外试验和斑马鱼胚胎的体内毒性评估.

主要成果:

  • 化合物3,与4,4'-dimethylbipyridyl替代,在几分钟内在癌细胞中显示出强大的线粒体膜脱极化.
  • 鲁II) 复合物3诱导线粒体脱极化增加了8倍,超过了CCCP的效果.
  • 化三酸联体保持了抗癌作用,同时降低了斑马鱼胚胎中的毒性.

结论:

  • 辅助配体显著影响Ru(II) 协调化合物的抗癌活性.
  • 能够诱导线粒体功能障碍的Ru (II) 化合物代表了癌症治疗的有希望的途径.
  • 开发的Ru(II) 化合物显示了针对性癌症治疗的潜力,并改善了安全性.