细胞膜定位光探针用于阿米诺酶N
Yun Jae Yang1, Mingchong Dai2, Kyo Han Ahn1
1Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, South Korea.
ACS sensors
|July 5, 2023
概括
新的探针检测到细胞膜上的aminopeptidase N (APN) 活性,克服了内部探针的局限性. 这使得可以在组织和疾病模型中准确测量APN水平,帮助诊断和研究.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 医疗成像医学成像
背景情况:
- 氨基酶N (APN) 是一种涉及细胞功能和疾病的跨膜酶.
- 在瘤和受损器官中观察到升高的APN水平,需要非侵入性检测方法.
- 现有的APN探针测量细胞内光,导致由于细胞透性和酶动力学导致不准确的数据.
研究的目的:
- 开发新的细胞膜定位探针,以准确检测APN活动.
- 为了解决目前用于APN监测的细胞内探针的局限性.
- 为了使生物组织和疾病模型中APN水平的可靠量化.
主要方法:
- 开发了两个小分子探针,可定位到细胞膜.
- 探测器在APN酶活性时产生比度光信号.
- 利用双光子成像来确定各种器官和疾病模型中的APN水平.
主要成果:
- 已开发的探头选择性地对APN作出反应,以比度光变化.
- 在小鼠器官中的量化相对APN水平:肠 (4.3), (2.1),肝 (2.7),肺 (3.2),胃 (1.0).
- 在HepG2-异种移植瘤和乙氨基诱导的肝损伤模型中观察到升高的APN水平.
结论:
- 新型探针为研究APN相关生物学提供了一种可靠的方法.
- 能够准确,非侵入性地评估组织和疾病状态中的APN水平.
- 促进研究APN在药物诱导肝毒性等疾病中的作用.
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