在Staphylococcus aureus和Staphylococcus agnetis之间水平转移可能的病原性染色体岛屿
Douglas D Rhoads1, Jeff Pummil1,2, Nnamdi S Ekesi1,3
1Program in Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, United States of America.
PloS one
|July 5, 2023
概括
发现了针对病原体Staphylococcus agnetis和Staphylococcus aureus的新型移动遗传元素 (MGE). 这些元素,潜在的新染色体岛屿 (CI),可能在严重的感染和宿主病原体进化中发挥作用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 兽医科学 兽医科学 兽医科学
背景情况:
- 葡萄球菌 (Staphylococcus agnetis) 是一种新兴的家禽病原体,以前与牛乳腺炎有关.
- 现有的病毒性基因分析没有解释S. agnetis的过渡到严重的家禽感染.
- 在中S. agnetis的病原性遗传基础仍然不清楚.
研究的目的:
- 确定负责家禽中S. agnetis病变的新型遗传决定因素.
- 针对感染的S. agnetis分离物特有的移动遗传元素 (MGE) 的特征.
- 调查这些MGE与其他葡萄球菌菌种的进化关系.
主要方法:
- 全基因组测序和分析来自感染的S. agnetis分离物.
- BLASTn和遗传学分析来比较MGE与已知的基因组序列.
- 移动遗传元素 (MGE) 及其潜在载体 (菌体) 的识别和表征.
主要成果:
- 在的S. agnetis分离物中发现了一种新型的15kbp,17-19基因移动遗传元素 (MGE) 家族.
- 这些MGE在每个基因组中的多个副本中被发现,并且在一些菌株中被葡萄球菌菌体向量化.
- 在黄金葡萄球菌基因组中也检测到了密切相关的MGE,这表明水平基因转移.
- 这些MGE代表了染色体群岛 (CIs) 的新家族,S. agnetis和S. aureus之间共享.
结论:
- 鉴定到的MGE/CI是特定于S. agnetis和S. aureus的肉分离物.
- 这些新元素可能有助于家禽中严重的S. agnetis感染的发病.
- 需要进一步的研究来阐明这些CI在宿主-病原体相互作用和疾病演变中的作用.
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