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Updated: Jul 24, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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使用化学交叉链接和层次分析解码细胞中的蛋白质动力学

Beirong Zhang1,2, Zhou Gong3, Lili Zhao1,2

  • 1CAS Key Labratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.

Angewandte Chemie (International ed. in English)
|July 5, 2023
PubMed
概括

我们开发了一种新的方法来研究细胞中的蛋白质动力学,使用化学交叉链接和计算分析. 这种方法揭示了详细的蛋白质结构和运动,进步了我们对细胞蛋白质功能的理解.

关键词:
阿尔法2折2是什么意思在现场交叉链接.质谱测量质量谱测量蛋白质动力学 蛋白质动力学蛋白质结构 蛋白质结构

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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