黑色素B通过激活ER压力来改善胃癌瘤的生长
Wanli Xue1, Rui Sun2, Zheng Hao1
1Department of General Surgery, Jiaozuo Hospital of Traditional Chinese Medicine, Jiaozuo 454000, China.
Tissue & cell
|July 5, 2023
概括
黑色素B (HB) 有效地抑制胃癌 (GC) 细胞活力和瘤生长,通过诱导细胞亡和细胞循环停止通过内质网膜 (ER) 应激激活. 这表明HB是胃癌的有前途的治疗药物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 胃癌 (GC) 是全球癌症相关死亡的主要原因.
- 黑色素B (HB) 已显示出作为抗癌治疗剂的潜力.
研究的目的:
- 为了研究菌素B (HB) 对胃癌 (GC) 细胞的抗癌作用.
- 阐明HB作用的潜在机制,重点关注内分泌网膜 (ER) 应激通路.
主要方法:
- 用不同度的HB (0-50μM) 治疗GC细胞.
- 使用MTT试验评估了细胞活力.
- 通过流式细胞计量分析了亡和细胞循环进展.
- 对ER应激蛋白 (IRE1,CHOP,GRP78) 和Bcl-2的表达是通过西部斑块和免疫组织化学测量.
主要成果:
- 乙型肝炎显著降低了GC细胞活力和瘤生长,以剂量依赖的方式.
- HB诱导GC细胞亡和G0/G1细胞周期停止.
- HB上调的ER压力标志物 (IRE1,CHOP,GRP78) 和下调的Bcl-2表达.
- 抑制ER压力部分逆转了HB对GC细胞的影响.
- 乙型肝炎治疗增加了caspase-3的表达,但没有增加caspase-8.
结论:
- 乙基素有效地抑制胃癌细胞的增殖和瘤的生长.
- 乙型肝炎通过激活ER应激通路来发挥其抗癌作用,从而导致细胞亡和细胞循环停止.
- 乙型肝炎作为胃癌治疗的潜在治疗药物显示出希望.
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