乳头纤维细胞和网状纤维细胞产生不同的微环境,这些微环境对血管生成产生不同的影响
Adèle Mauroux1, Pauline Joncour2, Noémie Brassard-Jollive3
1Center for Interdisciplinary Research in Biology (CIRB), College de France - CNRS, INSERM, Université PSL, 11 Place Marcelin Berthelot, Paris 75005, France; Institut de Génomique Fonctionnelle de Lyon (IGFL), ENS de Lyon, CNRS, Univ Lyon 1, 32-34 Avenue Tony Garnier, Lyon 69007, France; R&D Department, SILAB, ZI de la Nau, Saint Viance 19240, France; Sorbonne Université, Collège Doctoral, 15 rue de l'Ecole de Médecine, Paris 75006, France.
Acta biomaterialia
|July 5, 2023
概括
皮肤纤维细胞,乳头和网状细胞,保持不同的基因表达,并创建独特的细胞外基因矩阵 (ECM). 这项研究强调了它们在血管化中的特定作用,用于组织工程和皮肤建模.
科学领域:
- 组织工程和再生医学
- 皮肤微环境和血管化
- 细胞生物学和细胞外矩阵 (ECM)
背景情况:
- 乳头皮肤和网状皮肤表现出与纤维细胞亚群相关的独特的ECM和血管化模式.
- 了解这些纤维细胞的作用对于开发功能性组织工程皮肤至关重要.
- 之前的研究强调了微环境在组织工程中的重要性.
研究的目的:
- 在组织工程背景下,研究 papillary 和 reticular 纤维细胞对 matrix 生产和血管化的贡献.
- 评估细胞放大和高密度培养是否影响纤维细胞基因表达特征.
- 用3D模型分析分泌和ECM相关因素对血管生成的影响.
主要方法:
- RNA-seq差异性表达分析,以评估放大纤维细胞的基因表达特征.
- 无脚手架的细胞叶培养模仿本地微环境条件.
- 两个独立的3D血管生成试验 (纤维素凝和基于细胞表) 来评估分泌和ECM相关的因素.
主要成果:
- 纤维细胞基因表达特征,包括血管生成和母细胞体特征,在培养后保持不变.
- 乳头纤维细胞产生了一个亲血管性微环境,具有密集的,分支的血管网络.
- 网状纤维细胞产生了更多的结构性ECM组件,并支持了较小的分支,较大的血管.
结论:
- 皮肤纤维细胞亚群通过分泌和ECM结合的因素差异调节血管生成.
- 乳头和网状纤维细胞对于设计和建模皮肤微环境和血管化至关重要.
- 细胞生成的ECM发挥着重要作用,质疑水凝对组织工程的普遍相关性.
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