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Updated: Jul 22, 2026

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克洛斯特里迪奥伊德困难PCR Tcdb周期值预测了毒素EIA阳性,但不是感染的严重程度

Regan Mah1, Kerstin Locher2, Theodore S Steiner3

  • 1Faculty of Medicine, University of British Columbia, 899 W 12th Ave, Vancouver, BC V5Z 1M9, Canada.

Anaerobe
|July 5, 2023
PubMed
概括

实时PCR周期值 (CT) 可以预测Clostridioides difficile感染 (CDI) 毒素结果,但对预测疾病严重程度效果不佳. 这可能有助于减少不必要的毒素测定.

关键词:
克洛斯特里迪奥伊德斯困难菌循环_门时间影响力评估_毒素聚合酶_链_反应

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...