甲氧化组学揭示了哥伦比亚儿童的胚胎囊亚型混合感染
Gisela M Garcia-Montoya1, Ana L Galvan-Diaz2, Juan F Alzate1
1Centro Nacional de Secuenciación Genómica CNSG, Sede de Investigación Universitaria-SIU, Medellín, Colombia; Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Grupo Pediaciencias, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
概括
胚胎囊 sp. 的情况. 是一种常见的肠道寄生虫. 一种新的NGS方法在儿童中检测到了ST1,ST2和ST3亚型,在16%的病例中发现了混合感染.
科学领域:
- 医学寄生虫学 医学寄生虫学
- 分子生物学分子生物学
- 微生物组研究 微生物组研究
背景情况:
- 胚胎囊 sp. 的情况. 是一种普遍存在的人类肠道前列腺体,具有有争议的致病性.
- 关于它在疾病中的作用,存在相互矛盾的证据.
- 遗传学研究将胚胎囊基因型与各种病原性特征联系起来.
研究的目的:
- 为了应用一个元taxonomic下一代测序 (NGS) 方法用于胚胎细胞 sp. 检测. 检测. 在检测.
- 为了识别来自梅德林的儿童的细胞囊亚型和混合感染.
- 克服传统显微镜和PCR方法用于混合感染检测的局限性.
主要方法:
- 采用了一种元taxonomic NGS方法,针对真核细胞的SSU-rRNA基因 (V4区域).
- 采用经典的遗传学方法来识别亚型.
- 分析了来自梅德林日托中心的儿童的便样本.
主要成果:
- 胚胎囊 sp. 的情况. 在感染儿童的便样本中检测到.
- 已确认的亚型为ST1,ST2和ST3.
- 在16%的分析样本中发现混合感染 (ST1 + ST3).
结论:
- 对于检测Blastocystis sp.的NGS方法是有效的 和其亚型,包括混合感染.
- 在研究的儿科患者群体中存在ST1,ST2和ST3亚型.
- 在这个城市环境中,混合胚囊炎感染是值得注意的发现.
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