用于高效的细胞酶生产和基纤维素生物精炼的真菌菌株改进:目前的状况和未来的前景
Jie Yang1, Hou-Ru Yue1, Li-Ya Pan2
1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresource technology
|July 5, 2023
概括
细胞工厂的设计是为了高效地产生细胞酶酶,用于分解纤维素生物质 (LCB). 代谢工程和多组学方面的进步改善了酶生产,降低了可持续生物燃料和生物化学合成的成本.
科学领域:
- 生物技术是生物技术.
- 生物精炼是生物精炼的一种方式.
- 合成生物学 合成生物学
背景情况:
- 细胞生物质 (LCB) 是生物燃料和生物化学品的关键可持续碳来源.
- 真菌细胞分解酶对于LCB降解至关重要,但高生产成本是一个挑战.
- 丝状真菌是细胞酶生产的成熟平台.
研究的目的:
- 总结最近在利用真菌细胞工厂进行增强细胞酶生产方面的进展.
- 突出提高LCB生物提炼的经济可行性的战略.
- 强调代谢工程和多组学在优化真菌生物催化剂中的作用.
主要方法:
- 为真菌细胞工厂开发先进的基因组工程工具.
- 使用促进体工程和转录因子调制来操纵基因表达.
- 应用多omics分析来识别用于酶增强的新型遗传标.
- 研究细胞酶生产的转化调节机制.
主要成果:
- 改造的真菌菌株显示出提高了细胞酶生产效率.
- 基因组工程工具增强了真菌细胞工厂的能力.
- 多omics方法揭示了新的基因元素来提高酶产量.
- 了解翻译调节是进一步优化细胞酶输出的关键.
结论:
- 菌细胞工厂的综合菌株工程对于高效的LCB生物转化至关重要.
- 优化的真菌生物催化剂将从LCB中显著受益于可持续的生物生产.
- 通过代谢工程来降低酶生产成本对于LCB生物炼油厂至关重要.
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