Hsa_circ_0000129 knockdown通过准miR-485-3p/SGMS2通路来减弱 keloid 纤维细胞中的增殖和迁移
Zi Li1, Wenhui Zhang2, Heting Zhang3
1Department of Orthopedics & Plastic Surgery, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan 430015, Hubei, China.
概括
循环RNAcirc_0000129通过海绵化miR-485-3p和上调SGMS2.2,促进了 keloid 纤维细胞的增殖和迁移. 准这种途径可能为 keloid 治疗提供一种新的治疗策略.
科学领域:
- 分子生物学分子生物学
- 皮肤病学 皮肤病学
- 生物化学 生物化学
背景情况:
- 异常的循环RNA (circRNA) 功能与 keloid 形成有关,但其具体作用仍未得到充分研究.
- 已知促进癌症的circ_0000129在 keloid 病原发生中起着尚未阐明的作用.
研究的目的:
- 调查circ_0000129在 keloid 纤维细胞增殖和迁移中的作用和机制.
- 为了阐明circ_0000129,miR-485-3p和SGMS2在 keloid 形成中的相互作用.
主要方法:
- 使用定量实时PCR和西式斑点测试来评估基因和蛋白质的表达.
- 进行了包括CCK8,EDU,伤愈合和Transwell迁移在内的细胞测试.
- 用路西法酶报告者和RNA免疫沉降试验来确定分子相互作用.
主要成果:
- 在circ_0000129和SGMS2上调,而miR-485-3p在质组织和纤维细胞中下调.
- 沉默circ_0000129抑制了 keloid 纤维细胞的增殖和迁移.
- circ_0000129直接菌miR-485-3p,这反过来又抑制了SGMS2的表达.
结论:
- circ_0000129 作为 miR-485-3p 的分子海绵,缓解 SGMS2 的抑制,促进 keloid 纤维细胞的增殖和迁移.
- 在circ_0000129/miR-485-3p/SGMS2轴呈现一个潜在的治疗化物治疗的目标.
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