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对来自BVDV的非结构性蛋白5A在膜中的子使用偏差的分析

Mingyang Gao^1,2, Xuanye Yang^1,2, Yuhu Wu^1,2

¹Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou.

Journal of basic microbiology

|July 5, 2023

概括

同义符号的使用和转移RNA (tRNA) 的丰富性影响了牛病毒性腹病毒 (BVDV) 非结构蛋白5A (NS5A) 的膜基形成. 这种编码子偏差会影响蛋白质结构,影响病毒复制.

科学领域:

病毒学病毒学
分子生物学分子生物学
生物物理学的生物物理.

背景情况:

牛病毒性腹病毒 (BVDV) 非结构蛋白5A (NS5A) 对于病毒复制至关重要.
NS5A通过两性α螺旋结合到细胞膜中,形成病毒复制复合体的一部分.
同义代码子使用模式和tRNA丰度是影响蛋白质表达和功能的已知因素.

研究的目的:

为了研究NS5A和细胞tRNA丰富的同名密码体使用模式对其内平面膜的形成的影响.
探索BVDV NS5A核酸使用,代码偏差和基因型特异性模式之间的关系.
要了解由tRNA丰度影响的翻译速度如何影响NS5A膜的空间形状.

主要方法:

分析不同BVDV基因型的NS5A编码序列.
与NS5A膜 anchor形成相关的同名编码子使用模式的评估.
对细胞中总体tRNA丰度的分析,以与NS5A内的编码子位置相关联.

主要成果:

BVDV NS5A编码序列表现出基因型特定的同义编码子使用模式,以核酸使用为主.
观察到同名编码子使用偏差和NS5A内平面膜的空间形状之间存在显著的相关性.
在膜内特定的编码子位置发现了更高的tRNA丰度,这表明增加翻译速度在塑造其结构方面发挥了作用.

结论:

同义符号使用偏差在确定BVDV NS5A内平面膜的空间形状方面发挥着至关重要的作用.
细胞tRNA的丰富程度会影响翻译速度,这反过来又会影响NS5A膜的形成.
这些发现为我们提供了新的见解,了解编码子使用偏差如何对病毒蛋白的结构和功能方面作出贡献.

关键词:

牛病毒性腹病毒病毒基因型特定的模式模式.在平面内膜 anchor 在平面内膜 anchor 非结构性蛋白质5A 5A 同义词 codon 使用情况这是一个趋势的趋势.

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