微生物细胞形态和组成对不同频率的简单介质的射频加热的影响
Julian Espitia1, Davy Verheyen1, Dmytro S Kozak1,2
1BioTeC+-Chemical and Biochemical Process Technology and Control, KU Leuven, Gebroeders de Smetstraat 1, 9000, Gent, Belgium.
Scientific reports
|July 5, 2023
概括
像Listeria monocytogenes,Salmonella Typhimurium和Saccharomyces cerevisiae这样的微生物显著增加了水和盐溶液中的射频加热温度. 它们的存在影响了加热速度,这表明有针对性的射频应用的潜力.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 电磁学 电磁学 电磁学 电磁学
背景情况:
- 射频 (RF) 加热是食品加工和杀菌的关键技术.
- 了解微生物存在如何影响加热动态对于过程优化至关重要.
- 以前的研究还没有广泛探索微生物对简单介质射频加热行为的影响.
研究的目的:
- 为了研究Listeria monocytogenes,Salmonella Typhimurium和Saccharomyces cerevisiae对射频加热的影响.
- 分析不同频率 (27.0MHz和3.0MHz) 和介质 (Milli-Q水,盐溶液) 对微生物加热的影响.
- 为了确定这些条件下的射频加热是否会影响微生物细胞活力.
主要方法:
- 用特定微生物接种的消毒的Milli-Q水和盐溶液接受了射频处理.
- 在两个不同的频率 (27.0 ± 0.6 MHz 和 3.0 ± 0.02 MHz) 进行了30分钟的射频处理.
- 在整个射频加热过程中,监测温度变化和微生物细胞数量.
主要成果:
- 微生物的存在导致温度显著上升,高达54.9°C.
- 在任何测试条件下都没有观察到微生物细胞数量的显著减少.
- 变热速率因物种而异 (Salmonella Typhimurium ≤ Listeria monocytogenes ≤ Saccharomyces cerevisiae) 并且在27.0MHz通常更高,但在盐溶液和Saccharomyces cerevisiae中存在例外.
结论:
- 微生物成分,可能含有脂质和类甘油,影响RF加热动态.
- 盐度可以与微生物存在相互作用,影响加热行为.
- 这项研究提供了开发特定射频频率的基础数据,用于针对性地加热介质和产品,从而有可能提高灭菌或加工效率.
相关概念视频
Microbial Growth Media
44
Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
44
Physical Methods for Controlling Microbial Growth: Temperature
116
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
116
Factors Influencing Microbial Growth: Temperature
68
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
68
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
106
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
106
Key Techniques in Microbiology
98
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
98
Microbial Morphologies
53
Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
53


