在UTI诊断中UF-5000自动查系统的有用性
Héctor Toledo1, Sara G Punzón2, Guillermo Martín-Gutiérrez2
1Servicio de Microbiología Hospital Universitario Virgen del Rocío de Sevilla, Seville, Spain. hector.toledo.sspa@juntadeandalucia.es.
概括
赛斯麦克斯UF-5000细胞计可以查尿液中的细菌,其100 bact/μl的切断值显示出高灵敏度. 然而,对于较小的实验室来说,尿液查不具有成本效益.
科学领域:
- 临床微生物学 临床微生物学
- 医疗技术 医疗技术 医学技术
背景情况:
- 尿培养是诊断尿路感染的基石.
- 需要快速选方法来减少实验室的工作量和成本.
研究的目的:
- 评估Sysmex UF-5000自动化尿液分析仪,用于微生物学中选负尿液.
- 确定检测细菌和白细胞的最佳切断点.
- 评估不同实验室环境中尿液查的成本效益.
主要方法:
- 使用Sysmex UF-5000分析了3569个尿样.
- 细菌和白细胞的切断点的确定.
- 诊断准确性参数的计算 (灵敏度,特异性,PPV,NPV,AUC).
- 基于实验室工作负载的成本效益评估.
主要成果:
- 100个细菌/μl的切断值显示AUC为0.868,96%的灵敏度和50%的特异性,减少了40%的培养工作量.
- 积极预测值 (PPV) 为35.5%,负预测值 (NPV) 为95.4%.
- 来自细胞计的白细胞计数没有被发现对查有用.
结论:
- 赛斯麦克斯UF-5000可以有效地查尿液中的细菌,截止值为100 bact/μl,大大减少了对培养的需求.
- 使用这种方法进行尿液查对于每工作日处理尿液少于284个的实验室来说是不具有成本效益的.
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