增长激素治疗改善了青春期前羔羊的毛囊和卵细胞的发育

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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

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Kexiong Liu¹, Luyao Zhang¹, Qi Qi¹

¹State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Yuan-Ming-Yuan West Road, Haidian District, Beijing, 100193, China.

Journal of ovarian research

|July 5, 2023

概括

增长激素 (GH) 治疗改善了青春期前羔羊的超排卵反应和卵细胞发育能力. 这提高了胚胎生产效率,为改善年轻动物生殖结果提供了一个有希望的策略.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
发展生物学发展生物学

背景情况:

与成年绵羊相比,青春期前的羔羊对超排卵和较低的卵子细胞发育潜力表现出可变的卵巢反应.
众所周知,生长激素 (GH) 提高了人类和动物的生殖能力,但它对青春期前羔羊卵巢和卵细胞的具体影响尚不清楚.

研究的目的:

为了研究羊生长激素 (GH) 对卵巢反应和卵细胞发育能力的影响,在经过超级排卵的青春期前羔羊中.
探索GH在羔羊粒粉细胞,卵巢和卵泡液中作用的潜在分子机制.

主要方法:

青春期前 (4~6周大) 的羊羔在卵泡刺激激素 (FSH) 超排卵前和期间接受了高剂量 (50毫克) 或低剂量 (25毫克) 的绵羊GH治疗,短期 (2天) 或长期 (5天).
卵子被收集用于体外成熟和受精,并对其质量和发育能力进行评估.
通过颗粒细胞 (GC) 转录组,卵巢蛋白质组和卵泡液代谢组分析来探索机制.

主要成果:

用50毫克GH长期治疗增强了超排卵反应和卵细胞发育能力,增加了高质量的胚胎产量.
GH治疗改变了卵巢中的基因和蛋白质表达,减少了氧化应激,并促进了GCs的增殖,可能是通过PI3K/Akt信号传递.
毛囊液代谢组分析显示了抗氧化剂和脂肪酸的变化.

结论:

生长激素 (GH) 治疗对羔羊的卵巢功能产生积极影响,支持卵泡和卵细胞的发育.
GH的管理提高了前青春期羔羊的胚胎生产效率.
这项研究提供了GH作为年轻动物辅助生殖中有益的辅助剂的证据.

关键词:

毛囊液是什么? 毛囊液是什么?颗粒素细胞是颗粒素的细胞.生长激素生长激素的作用卵子细胞是卵子细胞.卵巢卵巢卵巢卵巢在青春期前的羔羊.

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