由LPS诱导的系统性炎症影响星细胞和微细胞与小鼠大脑皮层血管的动态相互作用
Evangelia Xingi1, Paraskevi N Koutsoudaki2, Irini Thanou2
1Light Microscopy Unit, Hellenic Pasteur Institute, 11521 Athens, Greece.
Cells
|July 6, 2023
概括
神经炎症通过改变神经细胞和微质与血管的相互作用来破坏神经血管单元. 这种动态变化会影响中枢神经系统的血脑屏障完整性和质细胞功能.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 神经炎症是一种神经炎症.
背景情况:
- 神经血管单元 (NVU) 对于中枢神经系统 (CNS) 功能至关重要.
- 神经炎症,涉及微质细胞和星球细胞,与神经退行性疾病有关.
- 了解NVU内的质细胞动态对于神经退行性疾病研究至关重要.
研究的目的:
- 实时监测周血管星细胞和微质细胞的形态和动态变化.
- 在生理和神经炎症条件下研究质与大脑血管的相互作用.
- 阐明质反应在神经炎症诱导的NVU功能障碍中的作用.
主要方法:
- 使用2光子激光扫描显微镜 (2P-LSM) 在转基因小鼠中进行静脉内成像.
- 通过脂多糖 (LPS) 给药诱导的全身神经炎症.
- 可视化了微质和星质与血管系统的动态相互作用 in vivo.
主要成果:
- 激活的周围血管星球细胞失去了与血管系统的密切接触,可能会损害血脑屏障 (BBB) 的完整性.
- 激活的微质细胞增加了与血管的身体接触.
- 这些质动态反应在LPS后4天达到峰值,并在8天内持续存在,表明恢复不完全.
结论:
- 神经炎症在NVU内显著改变了星系细胞和微质细胞的相互作用.
- 星球细胞血管附近的干扰可能会导致BBB分解.
- 持续的质细胞变化表明对NVU功能和中枢神经系统健康的长期影响.
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