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双阳性CD4⁺CD8⁺ (DP) T细胞在慢性C型肝炎中显示出明显的疲劳表型

Anna Maria Kochanowicz¹, Sylwia Osuch¹, Hanna Berak²

¹Department of Immunopathology of Infectious and Parasitic Diseases, Medical University of Warsaw, 02-106 Warsaw, Poland.

|July 6, 2023

概括

在慢性型肝炎 (CHC) 中,双阳性 (DP) T 细胞与单阳性 (SP) T 细胞相比显示出明显的疲劳标记. 这些T细胞枯竭表型在很大程度上甚至在成功治疗型肝炎病毒后也存在.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
细胞生物学细胞生物学

背景情况:

慢性型肝炎 (CHC) 与T细胞耗尽有关.
在CHC外周血液中观察到双阳性 (DP) (CD4+CD8+) T细胞的频率增加.
在CHC中DPT细胞的枯竭表型仍然不太清楚.

研究的目的:

在CHC患者中比较DP和单阳性 (SP) T细胞之间的疲劳表型.
评估成功的HCV治疗对DP和SPT细胞中抑制受体表达的影响.
在DP和SP群体中研究型肝炎病毒 (HCV) 特定T细胞的特征.

主要方法:

使用流式细胞计量来评估编程细胞死亡蛋白1 (PD-1) 和T细胞免疫球蛋白以及含有粘素域的分子-3 (Tim-3) 的表达.
在治疗前和治疗后六个月,从97名CHC患者收集了血液样本.
鉴定出HCV特异性T细胞,并对疲劳标记进行分析.

主要成果:

与CD8+SP T细胞相比,DP T细胞在治疗前和治疗后表现出明显更高的PD-1和较低的Tim-3表达,与CD4+SP T细胞相比,PD-1-Tim-3-细胞更少.
治疗后PD-1+Tim-3+DP T细胞减少,但整体疲劳表型持续存在.
与HCV特异性SPT细胞相比,HCV特异性DPT细胞表现出明显的枯竭特征,这些特征在治疗后很大程度上仍然存在.

结论:

与SP T细胞相比,CHC患者的DP T细胞表现出独特的疲劳表型.
成功的HCV治疗导致T细胞耗尽标志物发生一些变化,但DPT细胞的独特表型在很大程度上仍然存在.
对CHC中DPT细胞功能进行进一步的研究是有必要的.

关键词:

CD4+CD8+双阳性 (DP) T细胞美国地方检察长:DAA 在PD-1中使用PD-1.T细胞耗尽的情况.提摩太书三章提摩太书三章抗病毒治疗是一种抗病毒治疗.在C型肝炎病毒的感染中,

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