多克隆抗体抑制了关键的细胞分解性乳头细菌物种的生长
Sara M Tondini1, Roderick I Mackie1,2, Joshua C McCann1
1Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Frontiers in microbiology
|July 6, 2023
概括
多克隆抗体通过减少生长和基质消失,有效地抑制了病菌,Ruminococcus albus和Fibrobacter succinogenes的向细菌. 这些特异性抗体为修改肠微生物种群提供了一种有希望的方法.
科学领域:
- 流体微生物学 流体微生物学
- 免疫学 免疫学 免疫学
- 动物营养动物营养
背景情况:
- 修改肠微生物种群可以增强发酵并提高动物生产力.
- 向抗体为特定的细菌操纵提供了一个潜在的战略.
- 关于抗体对乳头细菌的疗效的知识是有限的.
研究的目的:
- 开发有效的多克隆抗体来对抗关键的细胞分解性乳头细菌.
- 评估这些抗体对向细菌生长和功能的抑制作用.
- 评估开发的抗体对非病菌的特异性.
主要方法:
- 对Ruminococcus albus 7 (抗RA7),Ruminococcus albus 8 (抗RA8) 和Fibrobacter succinogenes S85 (抗FS85) 的纯种植物产生了多克隆抗体.
- 抗体的有效性在体外测试,在含有纤维素的介质中使用不同的剂量和注射时间.
- 测量了细菌生长 (光学密度),基质消失,乙酸度和细胞活力 (活/死污点). 还评估了交叉反应性和蛋白质结合 (西式涂抹,LC-MS/MS).
主要成果:
- 高剂量的特异性抗体显著降低了最终的光密度,酸盐度和向物种的活细菌细胞 (P < 0.01).
- 在F. succinogenes S85培养物中,抗FS85抗体的添加至少减少了48%的基质消失 (P < 0.01).
- 抗体具有很高的特异性,对非向细菌物种或非细胞分解性细菌的影响很小,由西方污点和蛋白质分析证实.
结论:
- 来自蛋的多克隆抗体有效抑制向的细胞分解性乳头细菌的生长.
- 开发出来的抗体对它们的目标物种表现出特异性,最大限度地减少了非目标效应.
- 经过验证的多克隆抗体代表了针对性修改肠微生物种群的可行方法.
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