混合葡萄藤Vitis"Chambourcin"的基因组组合
Sagar Patel1,2,3, Zachary N Harris1,2, Jason P Londo4
1Saint Louis University, Department of Biology, 3507 Laclede Ave, St. Louis, MO 63103, USA.
GigaByte (Hong Kong, China)
|July 6, 2023
概括
研究人员已经对这些元素进行了测序.
科学领域:
- 植物基因组学 植物基因组学
- 葡萄种植 葡萄种植 葡萄种植
- 生物信息学是一种生物信息学.
背景情况:
- "Chambourcin"是一种重要的法美杂交葡萄品种,用于酒.
- 混合葡萄树的基因组资源有限,这阻碍了研究.
- 了解"Chambourcin"基因组对于葡萄酒的改进至关重要.
研究的目的:
- 为了组装和注释"Chambourcin"葡萄藤的基因组.
- 为未来的研究和育种提供必要的基因组资源.
- 为了使*Vitis*属内的比较基因组学成为可能.
主要方法:
- 使用了PacBio HiFi长读,Bionano光学映射和Illumina短读测序.
- 进行了基因组组装和基因模型预测.
- 与其他*Vitis*物种进行了比较分析,并确定了简单的序列重复.
主要成果:
- 创建了一个高质量的基因组组装,有26个支架和23.3Mb的N50.
- 预测了33,791个基因模型,并确定了16,056个与其他葡萄树的ortologs.
- 发现了1606种植物转录因子和304571个简单序列重复.
结论:
- "Chambourcin"基因组组合为葡萄藤基因组学提供了一个基础资源.
- 这项工作促进了功能性基因组研究和杂交葡萄的基因组辅助育种.
- 现有的基因组数据将促进葡萄种植和葡萄酒学方面的研究.
相关概念视频
03:36Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
02:40Chromosome Structure
22.9K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.9K
01:20Monohybrid Crosses
230.4K
Overview
230.4K
02:27Trihybrid Crosses
23.5K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
23.5K
01:57Sanger Sequencing
755.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
755.1K
01:44Complementary DNA
29.6K
Overview
29.6K