相关实验视频

Updated: Jul 24, 2025

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

IGF2-NR4A2信号调节巨细胞亚型,减轻肝硬化

Lichao Yao¹, Xue Hu¹, Mengqin Yuan¹

¹Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Journal of clinical and translational hepatology

|July 6, 2023

概括

将胰岛素样生长因子2 (IGF2) 与骨髓衍生巨细胞 (BMDMs) 结合起来,可以有效地治疗小鼠肝硬化. 这种组合疗法可以减少肝炎和纤维化,比单独使用BMDMs更能促进肝脏再生.

科学领域:

肝病学肝病学是一种肝病学.
免疫学免疫学免疫学
再生医学是一种再生医学.

背景情况:

肝硬化是一种严重的疾病,可以导致肝衰竭和死亡.
巨细胞在调节肝硬化中矩阵沉积和降解方面发挥着至关重要的作用.
基于巨细胞的细胞疗法是肝移植的潜在替代方案,但其有效性需要进一步验证.

研究的目的:

研究结合胰岛素样生长因子2 (IGF2) 与骨髓衍生巨细胞 (BMDMs) 治疗肝硬化病的治疗潜力.
在小鼠模型中评估IGF2和BMDM联合治疗对肝炎,纤维化,功能和再生的影响.

主要方法:

在小鼠中的CCl4诱导肝硬化模型.
评估肝炎,纤维化回归,肝功能和肝细胞增殖.
活化肝星细胞 (HSC) 与巨细胞在IGF2的存在或缺席的情况下进行体外共培养.
对巨细胞极性和HSC抑制的分析.
通过基因过度表达来验证IGF2的影响.

主要成果:

联合治疗IGF2和BMDM显著减少肝炎和纤维化.
与单独使用BMDM相比,IGF2 + BMDM治疗增强了肝细胞的增殖.
实验室研究表明,IGF2通过上调NR4A2来抑制HSC激活,从而促进抗炎性巨细胞的表型.
IGF2增加了矩阵金属蛋白酶 (MMP) 的巨细胞合成,有助于增强治疗效果.

结论:

结合IGF2和BMDMs为肝硬化提供了一个有前途的治疗策略.
这种方法为开发基于巨细胞的先进细胞疗法提供了理论基础.
进一步的研究可以探索IGF2-增强的巨细胞治疗肝脏疾病的临床应用.

关键词:

细胞疗法是一种细胞疗法.肝脏的星状细胞.IGF2 IGF2 的意思是什么肝硬化是肝硬化的一种疾病.巨细胞是一个巨细胞.

更多相关视频

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Published on: May 5, 2023

相关概念视频

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...