瘤治疗场的有效性,以减少脂质瘤细胞系的增殖和迁移
Won Seok Lee1, Yoonjung Jang2, Ahyeon Cho3
1Department of Biochemistry, School of Medicine, Daegu Catholic University, Daegu, Gyeongsangbuk-do 42472, Republic of Korea.
Experimental and therapeutic medicine
|July 6, 2023
概括
瘤治疗领域 (TTFields) 通过抑制癌细胞的增殖,活力和迁移,在治疗脂质肉瘤 (LPS) 中表现有前途. 这种新型疗法,特别是与多克索鲁比辛结合,会诱导亡,并可能成为未来临床试验的基础.
科学领域:
- 在瘤学瘤学.
- 生物物理学的生物物理.
- 癌症治疗方法 癌症治疗方法
背景情况:
- 脂质肉瘤 (LPS) 是一种罕见的软组织肉瘤,治疗策略不清楚.
- 瘤治疗场 (TTFields) 代表了抗瘤疗法的新方法.
- 以前的研究表明,TTFields与化学放射治疗相结合,可以提高疗效.
研究的目的:
- 评估TTFields在抑制脂质肉瘤细胞增殖和活力的有效性.
- 研究TTFields对LPS细胞迁移和细胞亡的影响.
- 探索TTFields与多克索鲁比辛 (DOX) 结合在LPS细胞上的协同效应.
主要方法:
- 用TTFields (150 kHz,1.0 V/cm) 对两个LPS细胞系 (94T778和SW872) 的处理.
- 使用的测试包括试蓝,MTT,Transwell室,Caspase-3活性和反应性氧物种 (ROS) 测试.
- 对结合的TTFields和DOX治疗对细胞迁移的评估.
主要成果:
- 在3D培养中,TTFields显著降低了LPS细胞活力,增殖和殖民地形成.
- TTFields治疗显著降低了LPS细胞迁移,并通过ROS生成和caspase-3激活增加了细胞亡.
- 与TTFields和DOX的联合治疗协同增强了ROS诱导的亡和抑制迁移.
结论:
- TTFields在抑制脂肉瘤细胞生长,迁移和诱导亡方面显示出显著的潜力.
- TTFields和多克索鲁比辛的组合显示出协同效应,增强了抗癌活性.
- 这些发现为研究TTFields作为临床环境中治疗脂肪肉瘤的治疗策略提供了基础.
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