在腰椎椎间板的循环颗粒细胞髓衍生抑制细胞的水平增加
Hui Zhou1, Chang Liu2, Fangfang Hu1
1Department of Physiology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Experimental and therapeutic medicine
|July 6, 2023
概括
骨髓衍生抑制细胞 (MDSCs) 与腰椎间盘 (LDH) 有关. 颗粒细胞-MDSCs (G-MDSCs) 随着LDH严重程度的增加而增加,这表明它们作为疾病进展的生物标志物的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 整形外科 整形外科 整形外科
- 细胞生物学 细胞生物学
背景情况:
- 众所周知,在炎症和慢性疾病期间,骨髓原抑制细胞 (MDSC) 会扩大.
- 在椎间盘退化 (IDD) 中MDSCs的具体作用尚不清楚.
- 腰椎间盘 (LDH) 是与IDD相关的常见疾病.
研究的目的:
- 调查特定的MDSC子集在腰椎间盘 (LDH) 中的作用.
- 确定MDSC子集是否可以作为LDH进展的指标.
- 分析循环MDSCs与LDH临床病理阶段之间的相关性.
主要方法:
- 对LDH中的粒细胞-MDSC (G-MDSC) 表达的基因表达综合 (GEO) 数据库的分析.
- 流式细胞计量用于对40名LDH患者和15名对照者的外周血液中MDSC子集的特征.
- 腰椎MRI用于评估疾病阶段 (Pfirrmann分级).
- 使用t分布式随机邻域嵌入和FlowSOM进行计算分析.
主要成果:
- 对GEO数据库的分析表明,LDH患者的G-MDSCs表达高.
- 循环G-MDSC频率与Pfirrmann阶段III和IV正相关.
- 单核MDSC (M-MDSC) 的百分比显示出一个上升的趋势.
- 年龄和性别与G-MDSC或M-MDSC频率没有显著的相关性.
结论:
- 腰椎间盘 (LDH) 改变了循环中的MDSC亚群.
- 增加的G-MDSC频率与LDH的晚期 (III和IV) 相关.
- 确定G-MDSC可以作为LDH的有价值的辅助诊断标记.
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