相关实验视频

Updated: Jul 24, 2025

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

NONHSAT098487.2通过调节Notch通路来保护心肌细胞免受氧化应激损伤

Guiju Feng¹, Hong Zhang¹, Qingling Guo²

¹Department of General Practice, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China.

|July 6, 2023

概括

一种新的长非编码RNA,NONHSAT098487.2,可以保护心脏细胞免受氧化应激. 这种RNA在急性心肌梗塞患者中被上调,并激活了Notch通路,为心脏损伤提供了潜在的治疗点.

科学领域:

心血管生物学心血管生物学
分子心脏病学分子心脏病学
非编码RNA研究研究

背景情况:

急性心肌梗塞 (AMI) 是全球心血管死亡的主要原因.
长非编码RNAs (lncRNAs) 与心血管疾病有关,但它们在保护心肌细胞免受氧化压力的作用尚不清楚.
反应性氧物种 (ROS) 在AMI中对心肌细胞损伤有显著的贡献.

研究的目的:

研究新型lncRNA NONHSAT098487.2在保护心肌细胞免受过氧化 (H2O2) 诱导的氧化应激作用中的功能.
探索涉及Notch信号通路的潜在分子机制.

主要方法:

定量实时聚合酶链反应 (qRT-PCR) 用于基因表达分析.
细胞活力测试 (细胞计数套件-8),乳酸脱酶 (LDH) 释放测试,以及用于评估亡的流细胞计量.
西部涂抹以评估蛋白质水平和Notch通路激活. 使用隙抑制剂 (DAPT) 来确认途径的参与.

主要成果:

在AMI患者的外周血液单核细胞中,NONHSAT098487.2表达升高,与心脏损伤生物标志物 (HS-TnT,CK-MB) 相相关.
NONHSAT098487.2在氧化应激 (H2O2) 或低氧/低氧化状态下在心肌细胞上调调.
Knockdown 的 NONHSAT098487.2 通过抑制 Notch 信号来加剧 H2O2 诱导的损伤,而它的过度表达通过激活 Notch 信号来保护细胞.

结论:

在心肌细胞中,lncRNA NONHSAT098487.2 证明了对氧化应激的保护作用.
NONHSAT098487.2通过调节Notch信号通路来发挥其保护作用.
这种新型的lncRNA代表了缓解急性心肌梗塞等疾病中心肌细胞损伤的潜在治疗标.

关键词:

剧烈的心肌梗塞急性心肌梗塞没有NONHSAT098487.2的缺口信号通道的信号通道.氧化应激伤害的伤害是氧化应激伤害.在 lncRNA 中,

更多相关视频

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

相关概念视频

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...