带介质干细胞诱导CD4T细胞中的记忆表型+
Ezgi Sengun1, Tim G A M Wolfs2, Valéry L E van Bruggen2
1Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Medical Center Nijmegen, Radboud Institute for Molecular Life Sciences, Nijmegen, Netherlands.
Frontiers in immunology
|July 6, 2023
概括
人类介质干细胞 (hMSC) 调节T细胞反应,促进中央记忆表型. 细胞接触和膜因子都对这种免疫调节作用在炎症治疗中至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
背景情况:
- 炎症是许多疾病的基础,这给治疗带来了挑战.
- 人类介质干细胞 (hMSC) 具有免疫调节和再生特性.
- 在T细胞上hMSC调节的精确机制,特别是记忆形成,需要进一步阐明.
研究的目的:
- 调查带介质干细胞 (UC-MSC) 对CD4+T细胞记忆形成和反应能力的影响.
- 阐明UC-MSC免疫调节的基础机制,包括细胞与细胞接触和膜因子的作用.
主要方法:
- 联合培养UC-MSC与T细胞 (激活的PBMC或纯化的CD4+T细胞) 使用各种方法 (透孔,直接接触,条件介质).
- 免疫分析和细胞因子分泌分析以评估T细胞动态.
- 研究了细胞与细胞接触和膜因子对于UC-MSC免疫调节的必要性.
主要成果:
- UC-MSC治疗将效能记忆T细胞转移到中央记忆表型.
- 这种转变是可逆的,UC-MSC原始细胞保留了反应能力.
- 来自UC-MSC的细胞与细胞接触和膜因子都对显著的免疫调节作用至关重要.
- 介质素-6 (IL-6) 和转化生长因子-β (TGF-β) 在UC-MSC的功能中发挥了部分作用.
结论:
- UC-MSC显著影响T细胞激活,增殖和成熟.
- UC-MSC对T细胞的免疫调节能力取决于共同培养条件,需要细胞与细胞接触和膜信号传递.
- UC-MSC代表了一种有前途的治疗途径,用于管理T细胞介导的炎症性疾病.
相关概念视频
01:23Cells of the Adaptive Immune Response
1.1K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
1.1K
01:19Mesenchymal Stem Cells
4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
01:22T Cell Activation and Clonal Selection
832
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
832
01:23Immunological Memory
687
Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
687
01:40Cell-mediated Immune Responses
69.1K
Overview
69.1K
01:19Multipotency of Hematopoietic Stem Cells
3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K