基于水凝的生物电子舌头用于评估发酵鱼的乌玛米味道
Jing Liu1, Yeon Kyung Cha2,3, Yoonji Choi4
1School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, Shandong Province, China.
ACS sensors
|July 6, 2023
概括
一种新的基于水凝的生物电子舌头增强了乌玛米检测的灵敏度和稳定性. 这种先进的传感器准确地评估了鱼类提取物等真实食品样本中的乌玛米强度.
科学领域:
- 生物电子传感器 生物电子传感器
- 味道受体技术 味道受体技术
- 食品科学与分析 食品科学与分析
背景情况:
- 使用乌玛米味觉受体的生物电子舌头显示出对食品分析的希望.
- 实际应用受到有限的稳定性和真实样本中的非特异性反应的阻碍.
研究的目的:
- 开发一种稳定且灵敏的基于水凝的生物电子舌头,用于乌玛米强度评估.
- 克服现有传感器在现实世界食品样本分析方面的局限性.
主要方法:
- 在碳纳米管场效应晶体管电极上固定T1R1金星 (乌玛米味觉受体).
- 将聚烯胺导电水凝膜混合在传感器表面,以改善受体环境.
- 使用物理吸附用于水凝杂交,确保水友性和生物相容性.
主要成果:
- 实现了低至1 fM的乌玛米物质的敏感检测.
- 对于单谷氨酸和二氨酸酸的广泛检测范围 (10-1510-2 M),覆盖了人类口味值.
- 显著减少非特异性结合,并表现出长期稳定性,可在鱼类提取物样本中检测.
结论:
- 基于水凝的生物电子舌头提供了增强的灵敏度,稳定性和减少非特异性结合.
- 开发的传感器适合在复杂的食品矩阵中准确评估umami强度.
- 为食品和饮料风味评价提供了一个有前途的平台.
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