小型非编码RNAs CjNC140和CjNC110的直接相互作用优化了Campylobacter jejuni的关键病原性表型的表达
Brandon Ruddell1,2, Alan Hassall1,3, Walter N Moss4
1Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University , Ames, Iowa, USA.
mBio
|July 6, 2023
概括
在Campylobacter jejuni中,小型非编码RNAs (sRNAs) CjNC110和CjNC140对抗病毒性表型. CjNC140抑制,而CjNC110激活,影响铁的吸收和殖民.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌病原体的产生
背景情况:
- 小型非编码RNAs (sRNAs) 对于细菌病原体的基因表达调节至关重要.
- 在 *Campylobacter jejuni* 中,sRNA 的功能是食物传播疾病的主要原因,在很大程度上是未知的.
- CjNC110是之前描述的sRNA,它调节了*C. jejuni*中的毒性.
研究的目的:
- 为了阐明sRNA CjNC140在*Campylobacter jejuni*中的功能.
- 为了研究sRNAs CjNC140和CjNC110.0之间的相互作用.
- 了解这些sRNAs在细菌病原和铁平衡中的作用.
主要方法:
- 基因失活和表型分析 (机动性,自凝聚,L-甲,自诱因-2,H2O2抵抗,殖民).
- RNA测序 (RNAseq) 和北方涂抹用于分析sRNA表达.
- 电泳运动转移试验 (EMSA) 证实直接sRNA相互作用.
- 用于sRNA保护和二次结构预测的计算分析.
主要成果:
- 失活CjNC140增强了运动性,自凝结,L-氨酸,自诱导-2的产生,H2O2耐药性和早期的殖,这表明它具有抑制作用.
- 对于大多数测试的表型,CjNC110和CjNC140表现出对立的调节作用.
- CjNC140和CjNC110的表达是反向相关的,这表明EMSA证实了直接相互作用.
- CjNC140积极调节*p19*,一个关键的铁吸收载体,是RyhB.的潜在同类.
结论:
- CjNC140和CjNC110作为一个具有对立功能的调节对,在*C. jejuni*中保持平衡.
- 这种sRNA调节网络与铁的吸收有关,这是C. jejuni*毒性的一个关键因素.
- 这些发现为*C. jejuni*的病理生物学和治疗干预的潜在目标提供了新的见解.
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