海洋微生物的长读元基因组学揭示了多样表达的二次代谢物
Ranran Huang1, Yafei Wang1, Daixi Liu2
1Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, China.
Microbiology spectrum
|July 6, 2023
概括
长读测序从未培养的海洋微生物中恢复了339个多样化的生物合成基因集群 (BGC). 这种方法揭示了微生物BGC及其表达,有助于发现新型化合物.
科学领域:
- 微生物基因组学和生物技术
- 大基因组学和生物信息学
- 海洋微生物生态学
背景情况:
- 微生物的二次代谢产物对于生态作用和生物技术应用至关重要.
- 短读测序限制了从未培养的细菌中获得的全长生物合成基因集群 (BGC) 的恢复和多样性评估.
- 访问未培养微生物的巨大遗传潜力,以发现新型化合物仍然是一个挑战.
研究的目的:
- 为了从黄海山湾的未培养微生物系中获取全长的BGC序列.
- 评估BGC在海洋环境中的多样性和功能表达.
- 证明长读序列和元转录组学对BGC发现和分析的有用性.
主要方法:
- 从Aoshan湾的海水样本的长期读取的元基因组测序.
- 基因组挖掘使用生物信息学工具来识别和组装BGCs.
- 基因转录分析以确定BGC和相关酶的基因表达模式.
主要成果:
- 成功地从多种多样的,大部分未培养的细菌和考古学科中恢复了339个主要是全长的BGCs.
- 在主要族群中鉴定出高度多样化的BGC,包括蛋白质细菌,细菌杆菌,酸性细菌杆菌,真菌微生物杆菌和"候选热等离子体".
- 转录基因组学揭示了30.1%的二次代谢基因被积极表达,为BGC的功能作用提供了洞察力.
结论:
- 长时间读取的元基因组测序对于从复杂的微生物群落中获得高质量,未碎片化的BGC是有效的.
- 长读测序和元转录基因组的结合提供了一个强大的方法来探索专业化代谢物的未充分利用的遗传储备.
- 这种综合方法提供了对BGC的功能表达的直接视图,促进了生物勘探和了解微生物适应.
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