海藻内生植物Clonostachys rosea作为素及其抗癌作用的替代来源
Ramalingam Parthasarathy1, Manjegowda Chandrika2, Damodaran Sruthi2
1Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India. sarathy20bioinfo@gmail.com.
Archives of microbiology
|July 6, 2023
概括
海洋藻类内性真菌Clonostachys rosea产生了抗癌化合物素的剩余. 这一发现为素生产提供了一个新的,高效的来源,可能有利于癌症研究和治疗.
科学领域:
- 海洋生物技术 海洋生物技术
- 自然产品化学 自然产品化学
- 癌症研究 癌症研究
背景情况:
- 居住在海洋藻类中的内菌是新型生物活性化合物的有希望的来源.
- 海洋绿藻 Chaetomorpha antennina 息在内菌中,具有潜在的制药应用.
- 素是一种天然存在的黄类化合物,以其强大的抗癌特性而闻名.
研究的目的:
- 为了从Chaetomorpha antennina中分离和识别内菌.
- 研究真菌代谢物对癌细胞的细胞毒性潜力.
- 探索从海洋内菌中获得的素的生产和抗癌活性.
主要方法:
- 使用序列分析对内菌的分子鉴定.
- 使用乙烯酸乙烯提取和净化真菌代谢物.
- 气体染色学-质谱学 (GC-MS),高性能薄层染色学 (HPTLC),液体染色学-质谱学 (LC-MS) 和核磁共振 (NMR) 用于化合物分析.
- 细胞毒性测定 (IC50测定) 和对MCF-7细胞的亡分析.
主要成果:
- 克洛诺斯塔克斯被确定为Chaetomorpha antennina的内生植物.
- C. rosea的乙烯酸提取物对MCF-7乳腺癌细胞表现出显著的细胞毒性.
- 通过C. rosea在酸盐介质中实现了高产量酸盐 (1050 mg/L) 的生产.
- 纯化真菌素显示出强烈的细胞毒性 (IC50 = 35.5 ± 0.6 μM),并在MCF-7细胞中诱导了DNA分裂和亡.
结论:
- 克洛诺斯塔克斯是一种新且高效的酸生产剩余来源.
- 海洋内菌提供了一种获得具有显著抗癌活性的素的新方法.
- 这项研究突出了海洋内植物在发现和生产有价值的生物活性化合物的治疗应用方面的潜力.
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