使用质谱成像 (MSI) 在皮肤等价物中的药物的空间分辨量
Cristina Russo1,2, Malcolm R Clench3
1Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK. C.Russo@mdx.ac.uk.
Methods in molecular biology (Clifton, N.J.)
|July 6, 2023
概括
定量质谱成像 (QMSI) 能够在组织中准确分析药物分布. 这项研究引入了一种新的微观探测技术,用于使用MALDI-MSI精确的3D皮肤模型药物定量.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 药理学 药理学是指药理学的学科.
背景情况:
- 矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 对于绘制组织中的分子有价值.
- 使用MALDI-MSI在组织中准确,空间分辨的药物定量仍然是一个重大挑战.
- 开发强大的定量质谱成像 (QMSI) 方法对于制药应用至关重要.
研究的目的:
- 开发和验证一种QMSI方法,用于在3D皮肤模型中绝对量化药物分布.
- 解决目前MALDI-MSI技术的局限性,用于精确的基于组织的药物测量.
- 建立一个全面的工作流程,用于对外源和内源物种的定量分析.
主要方法:
- 微观探测技术用于分析和内部标准沉积.
- 矩阵升华用于一致的矩阵应用.
- 使用先进的QMSI软件和专门的质谱成像设置.
- 应用于用于药物分布分析的3D皮肤模型.
主要成果:
- 在3D皮肤模型中,成功地实现了药物分布的绝对量化.
- 开发的QMSI方法证明了它的稳定性和准确性.
- 工作流程允许在组织结构内对药物物种进行无标签的映射和定量.
结论:
- 描述的微观探测和QMSI方法可以在3D组织模型中准确,空间分辨的药物定量.
- 这种技术提高了MALDI-MSI在制药研究和药物开发方面的能力.
- 该研究为复杂的生物系统中更复杂的定量成像分析提供了基础.
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