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Updated: Jul 24, 2025

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

用MALDI-2-TOF-MS进行负离子模式N-甘氨酸质谱成像

Jens Soltwisch^1,2, Bram Heijs³

¹Center for Proteomics & Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.

Methods in molecular biology (Clifton, N.J.)

|July 6, 2023

概括

这项研究提出了一种新方案,用于分析临床组织样本中的N-链 glycans (N-glycans),使用 Matrix 辅助激光消化/电离质谱成像与激光诱导的后电化 (MALDI-2-MSI). 这种方法是针对固定在甲中的,嵌入在中的组织进行了优化.

科学领域:

生物医学研究的研究.
分析化学是一种分析化学.
质谱测量质量谱测量

背景情况:

在各种生物过程和疾病状态中,N-链接的糖化 (N-甘氨酸) 起着至关重要的作用.
对直接从临床组织中获得的N-甘氨酸在现场分析对于了解它们在疾病中的作用至关重要.
现有的用于分析甲固定,嵌 (FFPE) 组织中的N-甘氨酸的方法有局限性.

研究的目的:

开发和描述一个标准化的样品制备协议,用于N-糖甘分析.
为了使FFPE组织截面能够使用带有激光诱导后离子化 (MALDI-2-MSI) 的矩阵辅助激光脱离/离子化质谱成像.
为了促进临床样本中N-甘氨酸的现场空间映射.

主要方法:

开发一种新的样本准备工作流程.
应用矩阵辅助激光脱/离子化质谱成像与激光诱导的后离子化 (MALDI-2-MSI).
从固定在甲,嵌入 (FFPE) 的组织段中分析N-甘氨酸.

主要成果:

为准备FFPE组织用于MALDI-2-MSI分析N-糖而建立了一个强大的协议.
成功地在现场检测和成像N-glycans直接从FFPE组织段.
该协议允许在组织上下文内对N-甘氨酸的空间定位.

结论:

描述的协议显著提高了在FFPE临床样本中分析N-甘氨酸的能力.
MALDI-2-MSI是一种强大的技术,用于在FFPE组织中进行空间分辨的N-glycan分析.
这种方法对生物标志物发现和病理学诊断应用具有前景.

关键词:

激光诱导的后电离.马尔迪二号 (Maldi-2) 是一个质谱仪成像成像质谱仪成像矩阵辅助的激光脱吸/电离.N-链接的糖基化.橄糖橄糖橄糖在组织上的酶性消化.

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