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工业酵母中的工程化发酵:连续培养作为选择改进菌株的工具

Thalita P Basso¹, Dielle P Procópio², Thais H C Petrin²

¹Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP 13418-900, Brazil.

Letters in applied microbiology

|July 6, 2023

概括

工程酵母现在发酵糖,用于可持续的第二代乙醇生产. 这一突破提升了基纤维素生物质的乙醇产量,推动了基于生物的经济.

科学领域:

生物技术是生物技术.
生物能源是生物能源.
微生物工程微生物工程

背景情况:

红细胞生物质是可持续生物能源的关键可再生资源.
麦片菌不能自然发酵糖糖,如糖,限制第二代乙醇的生产.
半纤维素衍生糖对高效的生物燃料转化构成挑战.

研究的目的:

为了设计一种强大的Saccharomyces cerevisiae菌株,能够发酵糖.
为了增强酵母利用林氏纤维素解酸盐中的糖的能力.
使用工程酵母来提高体积乙醇生产率.

主要方法:

使用CRISPR-Cas9基因编辑来引入来自Scheffersomyces stipitis的氧化还原性树脂酶路径.
工业S. cerevisiae菌株SA-1是用XYL1,XYL2和XYL3.3基因进行的.
工程菌株在化学静止器中经历了64天的适应性进化,在不断增加的稀释速率下.

主要成果:

进化菌株 (DPY06) 显示了改善的西洛斯消耗动力学.
与父菌株 (SA-1 XR/XDH) 相比,DPY06 的体积乙醇生产率提高了 35%.
在微空气条件下,在半纤维素化介质中评估了性能.

结论:

基因工程和适应性进化成功地创造了一种酵母菌株,用于高效的粉糖发酵.
开发的菌株提供了一个有前途的解决方案,以增加第二代乙醇生产的林氏纤维素原料.
这一进步支持向可持续的生物经济转型.

关键词:

这种植物是Saccharomyces cerevisiae.化学静电培养化学静电培养进化工程是关于进化的工程.工业应变菌株工业应变菌株一个Xylose,一个Xylose.

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