实用策略,用于水性清理组织的强大而廉价的成像
Rebecca M Williams1, Jordana C Bloom2, Cara M Robertus3
1BRC Imaging Facility, Institute of Biotechnology, Cornell University, Ithaca, New York.
Journal of microscopy
|July 6, 2023
概括
这项研究为安装清除的生物组织用于光谱显微镜提供了实际指导. 在烯酸中进行标准化安装,使大样本的常规,经济高效的宏观成像成为可能.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 光学显微镜的使用方法
- 组织制备 组织制备
背景情况:
- 光板显微镜适用于大型,光学清理的组织.
- 目前的组织安装方法复杂且不一致.
- 专有配方和粘合剂使组织制备复杂化.
研究的目的:
- 为在光学管中安装清除的组织提供实用建议.
- 为了标准化清除的生物样本的宏观成像.
- 为了降低成本和提高光板显微镜中的可复制性.
主要方法:
- 使用烯酸管用于组织安装,评估球形偏差.
- 开发用于光板对齐和评估的方法.
- 实施技术来区分光和自光,并去除工件.
主要成果:
- 烯酸带来了最小的球形偏差 (NA < 0.65).
- 标准化的安装程序提高了可重复性.
- 方法有效地减少像差分散射和条纹文物这样的文物.
结论:
- 标准化管安装为清除组织成像提供了一种常规且廉价的方法.
- 描述的方法提高了对大样本的光谱显微镜的可靠性.
- 这项工作促进了清除组织的强大3D细分分析.
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