饥饿与恒定的阳极电位相结合,会在电活性生物膜中触发细胞内电子储存
João Pereira1, Patrícia Neves2, Vivian Nemanic3
1Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA, Leeuwarden, the Netherlands; Environmental Technology, Wageningen University, Bornse Weilanden 9, P.O. Box 17, 6700 AA, Wageningen, the Netherlands.
Water research
|July 6, 2023
概括
在生物膜中研究了电子储存,通过控制电子供体和受体的访问. 批量养有利于细胞内储存,而在间歇性潜力下持续养则促进了EPS中的细胞外储存.
科学领域:
- 生物电化学系统 生物电化学系统
- 微生物的电化学
- 环境生物技术环境生物技术
背景情况:
- 微生物中的电子储存以多基酸盐 (PHA) 和细胞外聚合物质 (EPS) 的形式发生.
- 生物电化学系统 (BES) 使用电活性生物膜 (EABfs) 来存储电子,但对捐赠体养效应的研究不足.
研究的目的:
- 研究EABfs中的电子储存作为操作条件的函数.
- 确定电子供体养模式 (连续与批次) 和阳极电位 (恒定与间歇) 对PHA和EPS积累的影响.
主要方法:
- 在多种阳极潜力方案和酸盐养策略下培养EABfs.
- 同焦激光扫描显微镜 (CLSM) 和富里埃变换红外光谱 (FTIR) 用于评估电子储存.
- 图像处理以量化聚基酸盐 (PHB) 和细胞数量.
主要成果:
- 库伦比效率在25-82%之间,生物质产量在10-20%之间,这表明储存是一种消耗电子的过程.
- 在恒定的阳极电位下进行批量养,导致Geobacter的细胞内PHB储存,由能量增加和碳饥饿引发.
- 在间歇性阳极电位下的连续养导致了最高的EPS积累,表明从多余能量中进行细胞外储存.
结论:
- 操作条件显著引导微生物群体行为和电子储存机制在EABfs.
- 定制 BES 操作可以训练 EABfs 进行特定的生物转换,提高效率和优化.
- 了解电子存储通路对于设计先进的BES应用至关重要.
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