活跃的CRISPR-Cas12a在水友金属有机框架上:具有高稳定性和活性的纳米生物复合材料用于核酸检测
Zhirun Ji1, Bin Zhou2, Zhaoyang Shang1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.
Analytical chemistry
|July 6, 2023
概括
研究人员使用金属有机框架 (MOF) 稳定了CRISPR-Cas12a (一种生物传感工具). 由此产生的纳米生物复合物COM增强了酶的稳定性,并使超敏感的SARS-CoV-2RNA检测成为可能.
科学领域:
- 生物技术是生物技术.
- 纳米材料科学 科学 纳米材料科学
- 分子生物学分子生物学
背景情况:
- CRISPR-Cas12a是一种精确的生物传感方法.
- 卡斯12a的有限稳定性阻碍了其实际使用.
- 金属有机框架 (MOF) 提供保护性封装.
研究的目的:
- 为了提高CRISPR-Cas12a的稳定性和适用性.
- 开发一个强大的生物传感器用于核酸检测.
主要方法:
- 选各种MOF与Cas12a的兼容性.
- 使用MAF-7形成一个Cas12a-on-MOF (COM) 纳米生物复合材料.
- 在恶劣条件下测试COM的酶活性和稳定性.
- 使用COM.开发了一种SARS-CoV-2RNA检测试验.
主要成果:
- 性MAF-7被确定与Cas12a高度兼容.
- 该COM纳米生物复合材料保持了高的Cas12a活性,并表现出对热,盐和有机溶剂的耐受性.
- COM使得一种超敏感的SARS-CoV-2RNA检测试验能够实现,检测极限为1个副本.
- 这代表了第一个活跃的Cas12a纳米生物复合材料生物传感器,不需要外解构.
结论:
- 金属有机框架封装有效地稳定了CRISPR-Cas12a.
- 开发的COM纳米生物复合材料是超灵敏核酸检测的有希望的平台.
- 这一策略克服了Cas12a的稳定性限制,用于更广泛的生物传感应用.
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:53CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...