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用于微流体细胞培养系统的光学葡萄糖传感器

Stefanie Fuchs1, Veronika Rieger1, Anders Ø Tjell1

  • 1Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/II, 8010, Graz, Austria.

Biosensors & bioelectronics
|July 6, 2023
PubMed
概括

研究人员开发了一种用于微生理系统 (MPS) 的小型光学葡萄糖传感器. 这种插电式传感器能够在细胞培养物中稳定监测葡萄糖,解决了微流体系统的一个关键挑战.

关键词:
通过细胞流动.葡萄糖是一种葡萄糖.微流体的微流体微生理系统是微生理系统.这是一个光学传感器.器官在芯片上的器官

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科学领域:

  • 生物医学工程 生物医学工程
  • 传感器技术 传感器技术
  • 细胞培养技术 细胞培养技术

背景情况:

  • 葡萄糖对人类细胞能量至关重要,因此对其监测对于评估微生理系统 (MPS) 中细胞活力和代谢状态至关重要.
  • 目前在MPS中持续监测葡萄糖,由于缺乏适当的微型传感器解决方案而受到阻碍.

研究的目的:

  • 开发和描述一个小型化的,酶化的,光学葡萄糖传感器,用于集成到微流体系统中.
  • 创建一个插电式传感器系统,以便与现有的MPS轻松集成,并在细胞培养条件下可靠地测量葡萄糖.

主要方法:

  • 制造一个直径为1毫米的酶,光学葡萄糖传感器元件,与生物相容的带上的氧气传感器集成.
  • 在细胞培养条件下 (37°C,pH 7.4) 进行5天的传感器性能表征,评估氧气,pH,流量和杀菌等参数的漂移和影响.
  • 通过在静态细胞培养中进行在线葡萄糖测量来验证传感器系统,并将结果与商用葡萄糖传感器进行比较.

主要成果:

  • 在生理细胞培养条件下,开发的传感器在五天内表现出稳定的性能,轻微漂移 (3% day-1).
  • 该传感器系统与商用葡萄糖传感器相一致,可用于静态细胞培养中的在线葡萄糖测量.
  • 研究和记录了各种细胞培养参数对传感器性能的影响.

结论:

  • 一个易于集成的,小型化的光学葡萄糖传感器元件用于微流体系统已经成功开发.
  • 开发的传感器可以在细胞培养条件下进行稳定可靠的葡萄糖测量,解决了MPS研究中的关键需求.
  • 传感器系统的插即用性质使其在现有的MPS设置中更容易应用.