结合基于培养和分子方法,可以更好地评估沿海湖中的微生物质量
Marco Basili1, Laura Perini2, Luca Zaggia3
1CNR IRBIM, National Research Council - Institute of Marine Biological Resources and Biotechnologies, Largo Fiera della Pesca, 60125, Ancona, Italy.
Environmental pollution (Barking, Essex : 1987)
|July 6, 2023
概括
评估水中便污染需要多种方法. 将基于培养的技术与qPCR或高通量测序 (HTS) 结合起来,为管理污染风险提供了更好的数据.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生微生物学
- 水生生态学 水生生态学
背景情况:
- 水体中的便污染是全球公共卫生问题.
- 目前评估便污染的方法在可靠性和全面性方面缺乏共识.
研究的目的:
- 为了比较三个不同的方法在水环境中检测便污染的有效性.
- 评估每个方法及其组合提供的定量信息.
主要方法:
- 基于培养的方法进行便指标细菌 (FIB) 计数,针对FIB的qPCR试验和用于检测微生物种群的高通量测序 (HTS) 的比较.
- 一年来从模型湖和相邻海域抽取水和沉积物样本的分析.
主要成果:
- 所有方法都表明湖中的污染比海中的污染更高,沉积物中的污染比水中的污染更高.
- 便指标细菌 (FIB) 数量与培养和qPCR方法相关,qPCR产生更高的估计值.
- 便相关的细菌与培养的FIB相关,而污水相关的细菌只在水样中相关.
结论:
- 结合至少两种方法 (例如,培养和qPCR,或HTS) 提供了对便污染的增强质量和数量见解.
- 调查结果表明,不仅仅依赖FIB,还要将HTS纳入常规监测,以更好地管理便污染.
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