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在不同的环境场景下,日本 (Paralichthys olivaceus) 对变暖的应激反应
Ping Han1, Yingjie Qiao1, Jiayi He1
1Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, Shandong, China.
The Science of the total environment
|July 6, 2023
概括
由于全球变暖而导致的水温上升严重影响了日本鱼. 温度的突然升高会导致更多的肝损伤,氧化应激和内质网膜应激,而不是逐渐升高.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 环境科学 环境科学
- 鱼类生理学 鱼类生理学
背景情况:
- 日本 (Paralichthys olivaceus) 的养殖在亚洲至关重要.
- 全球变暖和极端天气事件威胁到水产养殖.
- 了解热应激对鱼类的影响对于行业的可持续性至关重要.
研究的目的:
- 调查日本鱼肝脏对热应激的组织学,亡,氧化应激和转录基因反应.
- 比较逐渐升温 (GTR) 和突然升温 (ATR) 的影响.
- 阐明这种经济重要鱼类物种中热应激背后的分子机制.
主要方法:
- 组织学检查和TUNEL染色以检测亡.
- 对血清和肝脏标记物的生物化学分析 (GPT,GOT,D-Glc,ATPase,糖原,TG,TC,ROS,SOD,CAT).
- RNA测序 (RNA-Seq) 用于转录基因分析和路径丰富分析 (KEGG,GSEA).
主要成果:
- 比起GTR,ATR会导致更严重的肝损伤 (空腔退行,炎症) 和亡.
- 与对照组相比,在GTR和ATR组中观察到显著的生物化学变化.
- RNA-Seq确定了GTR中的313个DEG和ATR中的644个DEG,其中包括细胞周期,蛋白质加工和DNA复制的关键途径受到影响.
- 细胞内膜网膜 (ER) 的压力标志物 (ATF4,JNK,CHOP,TRAF2) 被显著改变.
结论:
- 热应激会导致组织损伤,炎症,氧化应激和ER应激在日本鱼肝脏中.
- ATR比GTR构成更大的威胁,突出了养殖鱼类对快速环境变化的脆弱性.
- 调查结果提供了对面对水温上升的经济鱼类适应机制的见解.
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