热冲击蛋白TaHSP17.4,小麦中的TaHOP相互作用体,改善了植物的应激耐受性
Yi-Xuan Wang1, Tai-Fei Yu2, Chun-Xiao Wang3
1College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China; Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China.
International journal of biological macromolecules
|July 6, 2023
概括
小麦热冲击蛋白 (HSP),特别是TaHSP17.4和TaHOP,通过调节应激反应基因和改善生理条件,增强植物对干旱,盐和热应激的耐受性.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 压力反应应激反应
背景情况:
- 植物适应干旱和盐度等环境压力对于作物生产至关重要.
- 热冲击蛋白 (HSP) 是参与蛋白质平衡的重要分子伴侣,但它们在应激耐受性方面的确切作用尚未完全理解.
- 了解HSP功能可以提高作物弹性.
研究的目的:
- 识别和描述涉及小麦应力耐受性的热冲击蛋白质.
- 研究已识别的HSP之间的相互作用,以及它们在对压力的生理反应中的作用.
- 探索这些HSPs在提高小麦品种的潜力.
主要方法:
- 转录组分析以确定小麦中热应激诱导的基因.
- 酵母-两个混合测试以确定蛋白质相互作用.
- 植物中的候选基因过度表达,然后在压力条件下进行生理和分子分析.
- 定量实时PCR (qRT-PCR) 用于分析基因表达模式.
主要成果:
- 确定了热冲击蛋白TaHSP17.4,并发现干旱,盐和热应激显著诱导.
- TaHSP17.4与TaHOP相互作用,TaHOP是一种连接HSP70和HSP90.0的蛋白质.
- 过度表达TaHSP17.4和TaHOP增强了proline含量,降低了malondialdehyde水平,并对多种非生物压力产生了显著的耐受性.
- 与反应性氧物种清理和酸信号传递相关的应激反应基因在过度表达的植物中被上调.
结论:
- 在小麦对非生物压力的反应中,TaHSP17.4和TaHOP起着至关重要的作用.
- 这些蛋白质通过涉及改善生理状态和调节与压力相关的基因通路的机制来增强抗压能力.
- TaHSP17.4和TaHOP代表了开发耐压力小麦品种的有希望的候选基因.
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